117208 20240319080951.0 doi 10.3390/biomedicines10030709 sideral 128792 ART-2022-128792 eng Sánchez-Marco, J. (orcid)0000-0002-9023-741X Thioredoxin Domain Containing 5 Suppression Elicits Serum Amyloid A-Containing High-Density Lipoproteins 2022 Access copy available to the general public Unrestricted Thioredoxin domain containing 5 (TXNDC5) is a protein disulfide isomerase involved in several diseases related to oxidative stress, energy metabolism and cellular inflammation. In a previous manuscript, a negative association between fatty liver development and hepatic Txndc5 expression was observed. To study the role of TXNDC5 in the liver, we generated Txndc5-deficient mice. The absence of the protein caused an increased metabolic need to gain weight along with a bigger and fatter liver. RNAseq was performed to elucidate the putative mechanisms, showing a substantial liver overexpression of serum amyloid genes (Saa1, Saa2) with no changes in hepatic protein, but discrete plasma augmentation by the gene inactivation. Higher levels of malonyldialdehyde, apolipoprotein A1 and platelet activating factor-aryl esterase activity were also found in serum from Txndc5-deficient mice. However, no difference in the distribution of high-density lipoproteins (HDL)-mayor components and SAA was found between groups, and even the reactive oxygen species decreased in HDL coming from Txndc5-deficient mice. These results confirm the relation of this gene with hepatic steatosis and with a fasting metabolic derive remedying an acute phase response. Likewise, they pose a new role in modulating the nature of HDL particles, and SAA-containing HDL particles are not particularly oxidized. © 2022 by the authors. Licensee MDPI, Basel, Switzerland. info:eu-repo/grantAgreement/ES/CIBERObn/CB06-03-1012 info:eu-repo/grantAgreement/ES/DGA-FSE/B16-20R info:eu-repo/grantAgreement/ES/MICINN-ISCIIII-FEDER/PID2019-104915RB-I00 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 4.7 2022 PHARMACOLOGY & PHARMACY 68 / 278 = 0.245 2022 Q1 T1 BIOCHEMISTRY & MOLECULAR BIOLOGY 92 / 285 = 0.323 2022 Q2 T1 MEDICINE, RESEARCH & EXPERIMENTAL 54 / 136 = 0.397 2022 Q2 T2 0.897 2022 Medicine (miscellaneous) 2022 Q1 Biochemistry, Genetics and Molecular Biology (miscellaneous) 2022 Q2 3.7 2022 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Martínez-Beamonte, R. Universidad de Zaragoza (orcid)0000-0002-8100-5596 Diego, A. de Universidad de Zaragoza Herrero-Continente, T. Barranquero, C. (orcid)0000-0002-8442-8041 Arnal, C. Universidad de Zaragoza (orcid)0000-0003-1197-4276 Surra, J. Universidad de Zaragoza (orcid)0000-0002-5841-0462 Navarro, M. A. Universidad de Zaragoza (orcid)0000-0002-0108-1004 Osada, J. Universidad de Zaragoza (orcid)0000-0002-8251-8457 1012 410 Universidad de Zaragoza Dpto. Farmac.Fisiol.y Med.L.F. Área Fisiología 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 2008 700 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Producción Animal 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 1002 050 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Biología Celular 10, 3 (2022), 709 - [16 pp] Biomedicines 2227-9059 2377156 http://zaguan.unizar.es/record/117208/files/texto_completo.pdf Versión publicada 2821161 http://zaguan.unizar.es/record/117208/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:117208 articulos driver 2024-03-18-13:06:24 ARTICLE