000117219 001__ 117219
000117219 005__ 20230519145553.0
000117219 0247_ $$2doi$$a10.3389/fmicb.2021.657431
000117219 0248_ $$2sideral$$a126762
000117219 037__ $$aART-2021-126762
000117219 041__ $$aeng
000117219 100__ $$aBerini F.
000117219 245__ $$aAntimicrobial activity of nanoconjugated glycopeptide antibiotics and their effect on Staphylococcus Aureus biofilm
000117219 260__ $$c2021
000117219 5060_ $$aAccess copy available to the general public$$fUnrestricted
000117219 5203_ $$aIn the era of antimicrobial resistance, the use of nanoconjugated antibiotics is regarded as a promising approach for preventing and fighting infections caused by resistant bacteria, including those exacerbated by the formation of difficult-to-treat bacterial biofilms. Thanks to their biocompatibility and magnetic properties, iron oxide nanoparticles (IONPs) are particularly attractive as antibiotic carriers for the targeting therapy. IONPs can direct conjugated antibiotics to infection sites by the use of an external magnet, facilitating tissue penetration and disturbing biofilm formation. As a consequence of antibiotic localization, a decrease in its administration dosage might be possible, reducing the side effects to non-targeted organs and the risk of antibiotic resistance spread in the commensal microbiota. Here, we prepared nanoformulations of the ‘last-resort’ glycopeptides teicoplanin and vancomycin by conjugating them to IONPs via surface functionalization with (3-aminopropyl) triethoxysilane (APTES). These superparamagnetic NP-TEICO and NP-VANCO were chemically stable and NP-TEICO (better than NP-VANCO) conserved the typical spectrum of antimicrobial activity of glycopeptide antibiotics, being effective against a panel of staphylococci and enterococci, including clinical isolates and resistant strains. By a combination of different methodological approaches, we proved that NP-TEICO and, although to a lesser extent, NP-VANCO were effective in reducing biofilm formation by three methicillin-sensitive or resistant Staphylococcus aureus strains. Moreover, when attracted and concentrated by the action of an external magnet, NP-TEICO exerted a localized inhibitory effect on S. aureus biofilm formation at low antibiotic concentration. Finally, we proved that the conjugation of glycopeptide antibiotics to IONPs reduced their intrinsic cytotoxicity toward a human cell line. Copyright © 2021 Berini, Orlandi, Gamberoni, Martegani, Armenia, Gornati, Bernardini and Marinelli.
000117219 536__ $$9info:eu-repo/grantAgreement/EC/H2020/829162/EU/Redesigning biocatalysis: Thermal-tuning of one-pot multienzymatic cascades by nanoactuation/HOTZYMES$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 829162-HOTZYMES
000117219 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000117219 590__ $$a6.064$$b2021
000117219 592__ $$a1.314$$b2021
000117219 594__ $$a8.2$$b2021
000117219 591__ $$aMICROBIOLOGY$$b34 / 138 = 0.246$$c2021$$dQ1$$eT1
000117219 593__ $$aMicrobiology (medical)$$c2021$$dQ1
000117219 593__ $$aMicrobiology$$c2021$$dQ1
000117219 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000117219 700__ $$aOrlandi V.T.
000117219 700__ $$aGamberoni F.
000117219 700__ $$aMartegani E.
000117219 700__ $$0(orcid)0000-0002-2854-2907$$aArmenia, I.
000117219 700__ $$aGornati R.
000117219 700__ $$aBernardini G.
000117219 700__ $$aMarinelli F.
000117219 773__ $$g12 (2021), 657431 [18 pp]$$pFront. microbiol.$$tFrontiers in Microbiology$$x1664-302X
000117219 8564_ $$s5706516$$uhttps://zaguan.unizar.es/record/117219/files/texto_completo.pdf$$yVersión publicada
000117219 8564_ $$s2194645$$uhttps://zaguan.unizar.es/record/117219/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000117219 909CO $$ooai:zaguan.unizar.es:117219$$particulos$$pdriver
000117219 951__ $$a2023-05-18-15:52:36
000117219 980__ $$aARTICLE