000117252 001__ 117252
000117252 005__ 20240319080959.0
000117252 0247_ $$2doi$$a10.1128/spectrum.02294-21
000117252 0248_ $$2sideral$$a128754
000117252 037__ $$aART-2022-128754
000117252 041__ $$aeng
000117252 100__ $$0(orcid)0000-0001-7947-8813$$aMinjárez Sáenz, M.$$uUniversidad de Zaragoza
000117252 245__ $$aMining the Flavoproteome of Brucella ovis, the Brucellosis causing agent in Ovis aries
000117252 260__ $$c2022
000117252 5060_ $$aAccess copy available to the general public$$fUnrestricted
000117252 5203_ $$aFlavoproteins are a diverse class of proteins that are mostly enzymes and contain as cofactors flavin mononucleotide (FMN) and/or flavin adenine dinucleotide (FAD), which enable them to participate in a wide range of physiological reactions. We have compiled 78 potential proteins building the flavoproteome of Brucella ovis (B. ovis), the causative agent of ovine brucellosis. The curated list of flavoproteins here reported is based on (i) the analysis of sequence, structure and function of homologous proteins, and their classification according to their structural domains, clans, and expected enzymatic functions; (ii) the constructed phylogenetic trees of enzyme functional classes using 19 Brucella strains and 26 pathogenic and/or biotechnological relevant alphaproteobacteria together with B. ovis; and (iii) the evaluation of the genetic context for each entry. Candidates account for;2.7% of the B. ovis proteome, and 75% of them use FAD as cofactor. Only 55% of these flavoproteins belong to the core proteome of Brucella and contribute to B. ovis processes involved in maintenance activities, survival and response to stress, virulence, and/or infectivity. Several of the predicted flavoproteins are highly divergent in Brucella genus from revised proteins and for them it is difficult to envisage a clear function. This might indicate modified catalytic activities or even divergent processes and mechanisms still not identified. We have also detected the lack of some functional flavoenzymes in B. ovis, which might contribute to it being nonzoonotic. Finally, potentiality of B. ovis flavoproteome as the source of antimicrobial targets or biocatalyst is discussed. IMPORTANCE Some microorganisms depend heavily on flavin-dependent activities, but others maintain them at a minimum. Knowledge about flavoprotein content and functions in different microorganisms will help to identify their metabolic requirements, as well as to benefit either industry or health. Currently, most flavoproteins from the sheep pathogen Brucella ovis are only automatically annotated in databases, and only two have been experimentally studied. Indeed, certain homologues with unknown function are not characterized, and they might relate to still not identified mechanisms or processes. Our research has identified 78 members that comprise its flavoproteome, 76 of them flavoenzymes, which mainly relate to bacteria survival, virulence, and/or infectivity. The list of flavoproteins here presented allows us to better understand the peculiarities of Brucella ovis and can be applied as a tool to search for candidates as new biocatalyst or antimicrobial targets.
000117252 536__ $$9info:eu-repo/grantAgreement/ES/DGA/E35-20R$$9info:eu-repo/grantAgreement/ES/DGA/LMP13_21$$9info:eu-repo/grantAgreement/ES/MICINN-AEI/PID2019-103901GB-I00$$9info:eu-repo/grantAgreement/ES/MCIN/AEI/10.13039/501100011033
000117252 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000117252 590__ $$a3.7$$b2022
000117252 592__ $$a1.095$$b2022
000117252 591__ $$aMICROBIOLOGY$$b62 / 135 = 0.459$$c2022$$dQ2$$eT2
000117252 593__ $$aImmunology and Microbiology (miscellaneous)$$c2022$$dQ1
000117252 593__ $$aMicrobiology (medical)$$c2022$$dQ1
000117252 593__ $$aEcology$$c2022$$dQ1
000117252 593__ $$aPhysiology$$c2022$$dQ1
000117252 593__ $$aInfectious Diseases$$c2022$$dQ1
000117252 593__ $$aGenetics$$c2022$$dQ2
000117252 593__ $$aCell Biology$$c2022$$dQ2
000117252 594__ $$a2.4$$b2022
000117252 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000117252 700__ $$0(orcid)0000-0001-9047-0046$$aMartínez Júlvez, M.$$uUniversidad de Zaragoza
000117252 700__ $$0(orcid)0000-0003-3608-4720$$aYruela, I.
000117252 700__ $$0(orcid)0000-0001-8743-0182$$aMedina, M.$$uUniversidad de Zaragoza
000117252 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000117252 773__ $$g10, 2 (2022), e02294-21[32 pp.]$$pMicrobiol. spectr.$$tMicrobiology Spectrum$$x2165-0497
000117252 8564_ $$s7844761$$uhttps://zaguan.unizar.es/record/117252/files/texto_completo.pdf$$yVersión publicada
000117252 8564_ $$s2323869$$uhttps://zaguan.unizar.es/record/117252/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000117252 909CO $$ooai:zaguan.unizar.es:117252$$particulos$$pdriver
000117252 951__ $$a2024-03-18-13:59:41
000117252 980__ $$aARTICLE