000117254 001__ 117254 000117254 005__ 20240319081001.0 000117254 0247_ $$2doi$$a10.3389/fcimb.2022.887134 000117254 0248_ $$2sideral$$a128692 000117254 037__ $$aART-2022-128692 000117254 041__ $$aeng 000117254 100__ $$aComín, Jessica 000117254 245__ $$aThe MtZ Strain: Molecular Characteristics and Outbreak Investigation of the Most Successful Mycobacterium tuberculosis Strain in Aragon Using Whole-Genome Sequencing 000117254 260__ $$c2022 000117254 5060_ $$aAccess copy available to the general public$$fUnrestricted 000117254 5203_ $$aSince 2004, a tuberculosis surveillance protocol has been carried out in Aragon, thereby managing to detect all tuberculosis outbreaks that take place in the community. The largest outbreak was caused by a strain named Mycobacterium tuberculosis Zaragoza (MtZ), causing 242 cases as of 2020. The main objective of this work was to analyze this outbreak and the molecular characteristics of this successful strain that could be related to its greater transmission. To do this, we first applied whole-genome sequencing to 57 of the isolates. This revealed two principal transmission clusters and six subclusters arising from them. The MtZ strain belongs to L4.8 and had eight specific single nucleotide polymorphisms (SNPs) in genes considered to be virulence factors [ptpA, mc3D, mc3F, VapB41, pks15 (two SNPs), virS, and VapC50]. Second, a transcriptomic study was carried out to better understand the multiple IS6110 copies present in its genome. This allowed us to observe three effects of IS6110: the disruption of the gene in which the IS6110 is inserted (desA3), the overexpression of a gene (ppe38), and the absence of transcription of genes (cut1:Rv1765c) due to the recombination of two IS6110 copies. Finally, because of the disruption of ppe38 and ppe71 genes by an IS6110, a study of PE_PGRS secretion was carried out, showing that MtZ secretes these factors in higher amounts than the reference strain, thereby differing from the hypervirulent phenotype described for the Beijing strains. In conclusion, MtZ consists of several SNPs in genes related to virulence, pathogenesis, and survival, as well as other genomic polymorphisms, which may be implicated in its success among our population. 000117254 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/ 000117254 590__ $$a5.7$$b2022 000117254 592__ $$a1.308$$b2022 000117254 591__ $$aMICROBIOLOGY$$b28 / 135 = 0.207$$c2022$$dQ1$$eT1 000117254 593__ $$aMedicine (miscellaneous)$$c2022$$dQ1 000117254 591__ $$aIMMUNOLOGY$$b55 / 161 = 0.342$$c2022$$dQ2$$eT2 000117254 593__ $$aInfectious Diseases$$c2022$$dQ1 000117254 593__ $$aMicrobiology (medical)$$c2022$$dQ1 000117254 593__ $$aMicrobiology$$c2022$$dQ1 000117254 593__ $$aImmunology$$c2022$$dQ2 000117254 594__ $$a6.4$$b2022 000117254 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000117254 700__ $$aMadacki, Jan 000117254 700__ $$0(orcid)0000-0003-3154-0723$$aRabanaque, Isabel$$uUniversidad de Zaragoza 000117254 700__ $$0(orcid)0000-0002-9541-5609$$aZúñiga-Antón, María$$uUniversidad de Zaragoza 000117254 700__ $$aIbarz, Daniel$$uUniversidad de Zaragoza 000117254 700__ $$0(orcid)0000-0002-3312-7759$$aCebollada, Alberto 000117254 700__ $$0(orcid)0000-0003-2044-6782$$aViñuelas, Jesús$$uUniversidad de Zaragoza 000117254 700__ $$aTorres, Luis 000117254 700__ $$aSahagún, Juan$$uUniversidad de Zaragoza 000117254 700__ $$aKlopp, Christophe 000117254 700__ $$0(orcid)0000-0001-8841-6593$$aGonzalo-Asensio, Jesús$$uUniversidad de Zaragoza 000117254 700__ $$aBrosch, Roland 000117254 700__ $$0(orcid)0000-0003-2266-8602$$aIglesias, María-José$$uUniversidad de Zaragoza 000117254 700__ $$0(orcid)0000-0002-3011-2519$$aSamper, Sofía$$uUniversidad de Zaragoza 000117254 7102_ $$11011$$2630$$aUniversidad de Zaragoza$$bDpto. Microb.Ped.Radio.Sal.Pú.$$cÁrea Microbiología 000117254 7102_ $$13006$$2435$$aUniversidad de Zaragoza$$bDpto. Geograf. Ordenac.Territ.$$cÁrea Geografía Humana 000117254 7102_ $$11011$$2615$$aUniversidad de Zaragoza$$bDpto. Microb.Ped.Radio.Sal.Pú.$$cÁrea Medic.Prevent.Salud Públ. 000117254 773__ $$g12 (2022), 887134 [16 pp.]$$tFrontiers in Cellular and Infection Microbiology$$x2235-2988 000117254 8564_ $$s5040162$$uhttps://zaguan.unizar.es/record/117254/files/texto_completo.pdf$$yVersión publicada 000117254 8564_ $$s2442183$$uhttps://zaguan.unizar.es/record/117254/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000117254 909CO $$ooai:zaguan.unizar.es:117254$$particulos$$pdriver 000117254 951__ $$a2024-03-18-14:09:47 000117254 980__ $$aARTICLE