000117363 001__ 117363 000117363 005__ 20230519145534.0 000117363 0247_ $$2doi$$a10.3390/microorganisms9122627 000117363 0248_ $$2sideral$$a127007 000117363 037__ $$aART-2021-127007 000117363 041__ $$aeng 000117363 100__ $$0(orcid)0000-0001-5750-3643$$aPeris M.P.$$uUniversidad de Zaragoza 000117363 245__ $$aComparative study of real-time pcr (Taqman probe and sybr green), serological techniques (elisa, ifa and dat) and clinical signs evaluation, for the diagnosis of canine leishmaniasis in experimentally infected dogs 000117363 260__ $$c2021 000117363 5060_ $$aAccess copy available to the general public$$fUnrestricted 000117363 5203_ $$aCanine leishmaniasis (CanL) diagnosis is not fully resolved. Currently, two specific methodologies are in continuous development, the detection of the parasite DNA or RNA in target organs and the detection of specific antibodies against Leishmania sp. For a correct diagnosis, it has been shown that the joint use of this type of test is necessary. In this work, a Sybr Green and a TaqMan Probe based on real time PCRs (qPCR) was performed for the detection of Leishmania sp. in order to correlate the results with clinicopathological and serological evaluations (IFA, ELISA and DAT) to propose an optimal biological sample to be used to detect the parasite in both early and late stages of the infection. A total of four samples were processed: conjunctival swabs, popliteal lymph node aspirates, bone marrow aspirates, and peripheral blood from experimentally infected dogs belonging to a larger study. Our results indicated that a single non-invasive sample (conjunctival swab) and the application of both types of qPCR would be reliable for determining Leishmania infection as well as the disease stage in dogs, thus avoiding bone marrow, lymph node aspirate or blood samples collection. © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). 000117363 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/ 000117363 590__ $$a4.926$$b2021 000117363 592__ $$a0.862$$b2021 000117363 594__ $$a4.1$$b2021 000117363 591__ $$aMICROBIOLOGY$$b54 / 138 = 0.391$$c2021$$dQ2$$eT2 000117363 593__ $$aMicrobiology (medical)$$c2021$$dQ2 000117363 593__ $$aMicrobiology$$c2021$$dQ2 000117363 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000117363 700__ $$0(orcid)0000-0002-8282-5975$$aEsteban-Gil A. 000117363 700__ $$0(orcid)0000-0001-7331-2863$$aOrtega-Hernández P. 000117363 700__ $$0(orcid)0000-0001-9778-6545$$aMorales M.$$uUniversidad de Zaragoza 000117363 700__ $$0(orcid)0000-0002-4121-8087$$aHalaihel N.$$uUniversidad de Zaragoza 000117363 700__ $$0(orcid)0000-0002-2048-4749$$aCastillo J.A.$$uUniversidad de Zaragoza 000117363 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal 000117363 773__ $$g9, 12 (2021), 2627 [10 pp]$$pMicroorganisms$$tMicroorganisms$$x2076-2607 000117363 8564_ $$s523234$$uhttps://zaguan.unizar.es/record/117363/files/texto_completo.pdf$$yVersión publicada 000117363 8564_ $$s2860309$$uhttps://zaguan.unizar.es/record/117363/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000117363 909CO $$ooai:zaguan.unizar.es:117363$$particulos$$pdriver 000117363 951__ $$a2023-05-18-15:34:19 000117363 980__ $$aARTICLE