000117364 001__ 117364
000117364 005__ 20230519145534.0
000117364 0247_ $$2doi$$a10.3390/horticulturae7120527
000117364 0248_ $$2sideral$$a127008
000117364 037__ $$aART-2021-127008
000117364 041__ $$aeng
000117364 100__ $$aBielsa B.
000117364 245__ $$aGene expression analysis in cold stress conditions reveals BBX20 and CLO as potential biomarkers for cold tolerance in almond
000117364 260__ $$c2021
000117364 5060_ $$aAccess copy available to the general public$$fUnrestricted
000117364 5203_ $$aLate spring frosts can become one of the limiting factors for the expansion of cultivation area towards a harsher climate for the almond [Prunus amygdalus Batsch syn P. dulcis (Mill.) D.A. Webb] crop as spring frost can damage up to 90% of the harvest. In order to identify key genes favoring cold tolerance in almonds, branches from three late-blooming genotypes: ‘Guara’, ‘Soleta’ and ‘Belona’ were exposed at -4¿C during 24 h in a constant climate chamber. Phenotype analysis showed that ‘Guara’ and ‘Soleta’ had a greater acclimation capacity to cold than ‘Belona’. The qRT-PCR BioMark System technology was used to monitor the relative expression of 30 candidate genes with a potential relation to cold response, which are either involved in the ICE-CBF-COR pathway or the independent CBF pathway, and also genes not yet characterized or with unknown function in almond genome. Differences in the gene expression profiles were found among the three studied genotypes and the three time-points of cold exposure (0, 2 and 24 h). BBX20 and CLO genes behaved as differentiator genes between tolerant and susceptible genotypes in cold stress response in almond pistils. In addition, the differences of expression among the tolerant genotypes suggested the intervention of different mechanisms responding to cold stress in almonds. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
000117364 536__ $$9info:eu-repo/grantAgreement/ES/MICINN/RTI2018-094210-R100
000117364 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000117364 590__ $$a2.923$$b2021
000117364 592__ $$a0.468$$b2021
000117364 594__ $$a1.8$$b2021
000117364 591__ $$aHORTICULTURE$$b7 / 36 = 0.194$$c2021$$dQ1$$eT1
000117364 593__ $$aPlant Science$$c2021$$dQ1
000117364 593__ $$aHorticulture$$c2021$$dQ1
000117364 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000117364 700__ $$aÁvila-Alonso J.I.
000117364 700__ $$aMartí Á.F.I.
000117364 700__ $$aGrimplet J.
000117364 700__ $$0(orcid)0000-0002-3455-0145$$aRubio-Cabetas M.J.
000117364 773__ $$g7, 12 (2021), 527 [16 pp]$$pHorticulturae$$tHorticulturae$$x2311-7524
000117364 8564_ $$s8764987$$uhttps://zaguan.unizar.es/record/117364/files/texto_completo.pdf$$yVersión publicada
000117364 8564_ $$s2787738$$uhttps://zaguan.unizar.es/record/117364/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000117364 909CO $$ooai:zaguan.unizar.es:117364$$particulos$$pdriver
000117364 951__ $$a2023-05-18-15:34:23
000117364 980__ $$aARTICLE