000117382 001__ 117382
000117382 005__ 20230519145546.0
000117382 0247_ $$2doi$$a10.3390/microorganisms9081554
000117382 0248_ $$2sideral$$a127076
000117382 037__ $$aART-2021-127076
000117382 041__ $$aeng
000117382 100__ $$aEsteban-cuesta I.
000117382 245__ $$aPhenotypic and genetic comparison of a plant-internalized and an animal-isolated salmonella choleraesuis strain
000117382 260__ $$c2021
000117382 5060_ $$aAccess copy available to the general public$$fUnrestricted
000117382 5203_ $$aContamination of fresh produce with human pathogens poses an important risk for con-sumers, especially after raw consumption. Moreover, if microorganisms are internalized, no re-moval by means of further hygienic measures would be possible. Human pathogenic bacteria identified in these food items are mostly of human or animal origin and an adaptation to this new niche and particularly for internalization would be presumed. This study compares a plant-internalized and an animal-borne Salmonella enterica subsp. enterica serovar Choleraesuis aiming at the identification of adaptation of the plant-internalized strain to its original environment. For this purpose, a phenotypical characterization by means of growth curves under conditions resembling the indige-nous environment from the plant-internalized strain and further analyses using Pulsed-field gel electrophoresis and Matrix-assisted laser desorption ionization time of flight spectrometry were as-sessed. Furthermore, comparative genomic analyses by means of single nucleotide polymorphisms and identification of present/absent genes were performed. Although some phenotypical and genetic differences could be found, no signs of a specific adaptation for colonization and internaliza-tion in plants could be clearly identified. This could suggest that any Salmonella strain could directly settle in this niche without any evolutionary process being necessary. Further comparative analysis including internalized strains would be necessary to assess this question. However, these kinds of strains are not easily available. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
000117382 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000117382 590__ $$a4.926$$b2021
000117382 592__ $$a0.862$$b2021
000117382 594__ $$a4.1$$b2021
000117382 591__ $$aMICROBIOLOGY$$b54 / 138 = 0.391$$c2021$$dQ2$$eT2
000117382 593__ $$aMicrobiology (medical)$$c2021$$dQ2
000117382 593__ $$aMicrobiology$$c2021$$dQ2
000117382 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000117382 700__ $$0(orcid)0000-0001-9123-4037$$aLabrador M.
000117382 700__ $$aHunt K.
000117382 700__ $$aReese S.
000117382 700__ $$aFischer J.
000117382 700__ $$aSchwaiger K.
000117382 700__ $$aGareis M.
000117382 773__ $$g9, 8 (2021), 1554 [13 pp]$$pMicroorganisms$$tMicroorganisms$$x2076-2607
000117382 8564_ $$s13041762$$uhttps://zaguan.unizar.es/record/117382/files/texto_completo.pdf$$yVersión publicada
000117382 8564_ $$s2825108$$uhttps://zaguan.unizar.es/record/117382/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000117382 909CO $$ooai:zaguan.unizar.es:117382$$particulos$$pdriver
000117382 951__ $$a2023-05-18-15:44:17
000117382 980__ $$aARTICLE