000117388 001__ 117388 000117388 005__ 20240319080952.0 000117388 0247_ $$2doi$$a10.1007/s13105-022-00897-2 000117388 0248_ $$2sideral$$a128613 000117388 037__ $$aART-2022-128613 000117388 041__ $$aeng 000117388 100__ $$0(orcid)0000-0001-5573-6144$$aLayunta, Elena$$uUniversidad de Zaragoza 000117388 245__ $$aIntestinal serotonergic system is modulated by Toll-like receptor 9 000117388 260__ $$c2022 000117388 5060_ $$aAccess copy available to the general public$$fUnrestricted 000117388 5203_ $$aIntestinal serotonergic system is a key modulator of intestinal homeostasis; however, its regulation is still unclear. Toll-like receptor 9 (TLR9), an innate immune receptor, detects different external agents in the intestine, preserving intestinal integrity. Since little is known about TLR9 role in the intestine, our aim was to address the potential regulation between TLR9 and intestinal serotonergic system. Caco-2/TC7 cell line and intestinal tract of Tlr9−/− mice were used in this study. Serotonin uptake studies were performed, and molecular expression of different serotonergic components was analyzed by western blot and real-time PCR. Our results show that TLR9 activation inhibits serotonin transporter activity and expression, involving p38/MAPK and ERK/MAPK intracellular pathways, and reciprocally, serotonin increases TLR9 expression. Supporting this interaction, serotonin transporter, serotonin receptors and serotonin producer enzymes were found altered in intestinal tract of Tlr9−/− mice. We conclude that TLR9 could contribute to intestinal homeostasis by modulation of intestinal serotonergic system. 000117388 536__ $$9info:eu-repo/grantAgreement/ES/DGA/ARAINF-012-2008$$9info:eu-repo/grantAgreement/ES/DGA/B022-13$$9info:eu-repo/grantAgreement/ES/MICINN-FEDER/BFU2010-18971 000117388 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/ 000117388 590__ $$a3.4$$b2022 000117388 592__ $$a0.845$$b2022 000117388 591__ $$aPHYSIOLOGY$$b25 / 79 = 0.316$$c2022$$dQ2$$eT1 000117388 593__ $$aBiochemistry$$c2022$$dQ2 000117388 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b158 / 285 = 0.554$$c2022$$dQ3$$eT2 000117388 593__ $$aPhysiology$$c2022$$dQ2 000117388 593__ $$aMedicine (miscellaneous)$$c2022$$dQ2 000117388 594__ $$a7.5$$b2022 000117388 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000117388 700__ $$0(orcid)0000-0002-5797-3909$$aLatorre, Eva$$uUniversidad de Zaragoza 000117388 700__ $$0(orcid)0000-0002-5306-9365$$aGrasa, Laura$$uUniversidad de Zaragoza 000117388 700__ $$0(orcid)0000-0002-2114-0577$$aArruebo, María Pilar$$uUniversidad de Zaragoza 000117388 700__ $$0(orcid)0000-0002-8467-0356$$aBuey, Berta$$uUniversidad de Zaragoza 000117388 700__ $$0(orcid)0000-0002-9935-927X$$aAlcalde, Ana I. 000117388 700__ $$0(orcid)0000-0003-4758-3998$$aMesonero, José E.$$uUniversidad de Zaragoza 000117388 7102_ $$11012$$2410$$aUniversidad de Zaragoza$$bDpto. Farmac.Fisiol.y Med.L.F.$$cÁrea Fisiología 000117388 7102_ $$11002$$2050$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Biología Celular 000117388 773__ $$g78 (2022), 689–701$$pJ. physiol. biochem.$$tJournal of Physiology and Biochemistry$$x1138-7548 000117388 8564_ $$s1890628$$uhttps://zaguan.unizar.es/record/117388/files/texto_completo.pdf$$yVersión publicada 000117388 8564_ $$s2201465$$uhttps://zaguan.unizar.es/record/117388/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000117388 909CO $$ooai:zaguan.unizar.es:117388$$particulos$$pdriver 000117388 951__ $$a2024-03-18-13:08:25 000117388 980__ $$aARTICLE