117422 20250120150147.0 doi 10.3390/foods11030298 sideral 128249 ART-2022-128249 eng Tejedor-Calvo, E. (orcid)0000-0001-5635-413X Application of pressurized liquid extractions to obtain bioactive compounds from Tuber aestivum and Terfezia claveryi 2022 Access copy available to the general public Unrestricted A PLE (pressurized liquid extraction) method was adjusted following a full-factorial experimental design to obtain bioactive-enriched fractions from Tuber aestivum and Terfezia claveryi. Temperature, time and solvent (water, ethanol and ethanol–water 1:1) parameters were investi-gated. The response variables investigated were: obtained yield and the levels of total carbohydrate (compounds, ß-glucans, chitin, proteins, phenolic compounds and sterols). Principal component analysis indicated water solvent and high temperatures as more adequate parameters to extract polysaccharide-rich fractions (up to 68% of content), whereas ethanol was more suitable to extract fungal sterols (up to 12.5% of content). The fractions obtained at optimal conditions (16.7 MPa, 180¿ C, 30 min) were able to protect Caco2 cells from free radical exposure, acting as antioxidants, and were able to reduce secretion of pro-inflammatory cytokines in vitro: IL-6 (50%), and TNFa (80% only T. claveryi ethanol extract), as well as reduce high inhibitory activity (T. aestivum IC50: 9.44 mG/mL). © 2022 by the authors. Licensee MDPI, Basel, Switzerland. info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 5.2 2022 FOOD SCIENCE & TECHNOLOGY 34 / 142 = 0.239 2022 Q1 T1 0.771 2022 Food Science 2022 Q1 Health Professions (miscellaneous) 2022 Q1 Plant Science 2022 Q1 Microbiology 2022 Q2 Health (social science) 2022 Q2 5.8 2022 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion García-Barreda, S. Universidad de Zaragoza (orcid)0000-0002-7248-234X Sánchez, S. Universidad de Zaragoza (orcid)0000-0002-6382-8142 Morte, A. Siles-Sánchez, M. N. Soler-Rivas, C. Santoyo, S. Marco, P. Universidad de Zaragoza (orcid)0000-0003-3384-7534 4013 210 Universidad de Zaragoza Dpto. Didácticas Específicas Área Didáctica Ciencias Socia. 2008 640 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Nutrición Bromatología 5011 500 Universidad de Zaragoza Dpto. CC.Agrar.y Medio Natural Area Ingeniería Agroforestal 11, 3 (2022), 298 [19 pp] Foods Foods 2304-8158 1910258 http://zaguan.unizar.es/record/117422/files/texto_completo.pdf Versión publicada 2830688 http://zaguan.unizar.es/record/117422/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:117422 articulos driver 2025-01-20-14:59:45 ARTICLE