117449 20240319080959.0 doi 10.1016/j.isci.2022.103822 sideral 128128 ART-2022-128128 eng García-Mendívil, Laura Universidad de Zaragoza (orcid)0000-0002-2954-1068 Analysis of age-related left ventricular collagen remodeling in living donors: Implications in arrhythmogenesis 2022 Access copy available to the general public Unrestricted Age-related fibrosis in the left ventricle (LV) has been mainly studied in animals by assessing collagen content. Using second-harmonic generation microscopy and image processing, we evaluated amount, aggregation and spatial distribution of LV collagen in young to old pigs, and middle-age and elder living donors. All collagen features increased when comparing adult and old pigs with young ones, but not when comparing adult with old pigs or middle-age with elder individuals. Remarkably, all collagen parameters strongly correlated with lipofuscin, a biological age marker, in humans. By building patient-specific models of human ventricular tissue electrophysiology, we confirmed that amount and organization of fibrosis modulated arrhythmia vulnerability, and that distribution should be accounted for arrhythmia risk assessment. In conclusion, we characterize the age-associated changes in LV collagen and its potential implications for ventricular arrhythmia development. Consistency between pig and human results substantiate the pig as a relevant model of age-related LV collagen dynamics. © 2022 The Author(s) info:eu-repo/grantAgreement/ES/DGA-FSE/T39-20R-BSICoS group info:eu-repo/grantAgreement/ES/DGA/LMP128_21 info:eu-repo/grantAgreement/ES/DGA/LMP94_21 info:eu-repo/grantAgreement/EUR/ERC-2014-StG-638284 info:eu-repo/grantAgreement/ES/MICINN-FEDER/PID2019-105674RB-I00 info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 5.8 2022 MULTIDISCIPLINARY SCIENCES 15 / 73 = 0.205 2022 Q1 T1 1.624 2022 Multidisciplinary 2022 Q1 6.9 2022 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Pérez-Zabalza, María (orcid)0000-0002-3194-7796 Mountris, Konstantinos (orcid)0000-0003-2946-3044 Duwé, Sam Smisdom, Nick Pérez, Marta Universidad de Zaragoza (orcid)0000-0002-8133-2124 Luján, Lluis Universidad de Zaragoza (orcid)0000-0002-2053-9842 Wolfs, Esther Driesen, Ronald B. Vallejo-Gil, José María Fresneda-Roldán, Pedro Carlos Fañanás-Mastral, Javier Vázquez-Sancho, Manuel Matamala-Adell, Marta Universidad de Zaragoza (orcid)0000-0001-7482-2582 Sorribas-Berjón, Juan Fernando Bellido-Morales, Javier André Mancebón-Sierra, Francisco Javier Vaca-Núñez, Alexander Sebastián Ballester-Cuenca, Carlos Universidad de Zaragoza (orcid)0000-0002-2225-308X Oliván-Viguera, Aida (orcid)0000-0001-5348-924X Diez, Emiliano Ordovás, Laura (orcid)0000-0003-3982-1263 Pueyo, Esther Universidad de Zaragoza (orcid)0000-0002-1960-407X 1001 025 Universidad de Zaragoza Dpto. Anatom.,Embri.Genét.Ani. Área Anatom.Anatom.Patológ.Com 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 1013 090 Universidad de Zaragoza Dpto. Cirugía Área Cirugía 5008 800 Universidad de Zaragoza Dpto. Ingeniería Electrón.Com. Área Teoría Señal y Comunicac. 25, 2 (2022), 103822 [20 pp] iScience ISCIENCE 2589-0042 3935819 http://zaguan.unizar.es/record/117449/files/texto_completo.pdf Versión publicada 1399899 http://zaguan.unizar.es/record/117449/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:117449 articulos driver 2024-03-18-14:01:13 ARTICLE