000117453 001__ 117453
000117453 005__ 20240319081000.0
000117453 0247_ $$2doi$$a10.3390/ijms23084172
000117453 0248_ $$2sideral$$a128762
000117453 037__ $$aART-2022-128762
000117453 041__ $$aeng
000117453 100__ $$aAbuobeid, R.
000117453 245__ $$aSqualene through its post-squalene metabolites is a modulator of hepatic transcriptome in rabbits
000117453 260__ $$c2022
000117453 5060_ $$aAccess copy available to the general public$$fUnrestricted
000117453 5203_ $$aSqualene is a natural bioactive triterpene and an important intermediate in the biosynthesis of sterols. To assess the effect of this compound on the hepatic transcriptome, RNA-sequencing was carried out in two groups of male New Zealand rabbits fed either a diet enriched with 1% sunflower oil or the same diet with 0.5% squalene for 4 weeks. Hepatic lipids, lipid droplet area, squalene, and sterols were also monitored. The Squalene administration downregulated 9 transcripts and upregulated 13 transcripts. The gene ontology of transcripts fitted into the following main categories: transporter of proteins and sterols, lipid metabolism, lipogenesis, anti-inflammatory and anti-cancer properties. When the results were confirmed by RT-qPCR, rabbits receiving squalene displayed significant hepatic expression changes of LOC100344884 (PNPLA3), GCK, TFCP2L1, ASCL1, ACSS2, OST4, FAM91A1, MYH6, LRRC39, LOC108176846, GLT1D1 and TREH. A squalene-enriched diet increased hepatic levels of squalene, lanosterol, dihydrolanosterol, lathosterol, zymostenol and desmosterol. Strong correlations were found among specific sterols and some squalene-changed transcripts. Incubation of the murine AML12 hepatic cell line in the presence of lanosterol, dihydrolanosterol, zymostenol and desmosterol reproduced the observed changes in the expressions of Acss2, Fam91a1 and Pnpla3. In conclusion, these findings indicate that the squalene and post-squalene metabolites play important roles in hepatic transcriptional changes required to protect the liver against malfunction. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
000117453 536__ $$9info:eu-repo/grantAgreement/ES/CIBERObn/CB06/03/0021$$9info:eu-repo/grantAgreement/ES/CIBERObn/CB06-03-1012$$9info:eu-repo/grantAgreement/ES/DGA-FSE/B16-20R$$9info:eu-repo/grantAgreement/ES/MICINN-ISCIIII-FEDER/PID2019-104915RB-I00$$9info:eu-repo/grantAgreement/ES/MICINN/RTI2018-098113-B100$$9info:eu-repo/grantAgreement/EUR/SUDOE/INTERREG/Redvalue-SOE1-PI-E0123
000117453 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000117453 590__ $$a5.6$$b2022
000117453 592__ $$a1.154$$b2022
000117453 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b66 / 285 = 0.232$$c2022$$dQ1$$eT1
000117453 593__ $$aMedicine (miscellaneous)$$c2022$$dQ1
000117453 591__ $$aCHEMISTRY, MULTIDISCIPLINARY$$b52 / 178 = 0.292$$c2022$$dQ2$$eT1
000117453 593__ $$aPhysical and Theoretical Chemistry$$c2022$$dQ1
000117453 593__ $$aComputer Science Applications$$c2022$$dQ1
000117453 593__ $$aInorganic Chemistry$$c2022$$dQ1
000117453 593__ $$aSpectroscopy$$c2022$$dQ1
000117453 593__ $$aOrganic Chemistry$$c2022$$dQ1
000117453 593__ $$aMolecular Biology$$c2022$$dQ2
000117453 593__ $$aCatalysis$$c2022$$dQ2
000117453 594__ $$a7.8$$b2022
000117453 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000117453 700__ $$0(orcid)0000-0002-9023-741X$$aSánchez-Marco, J.
000117453 700__ $$aFelices, M. J.
000117453 700__ $$0(orcid)0000-0003-1197-4276$$aArnal, C.$$uUniversidad de Zaragoza
000117453 700__ $$aBurillo, J. C.
000117453 700__ $$aLasheras, R.
000117453 700__ $$aBusto, R.
000117453 700__ $$aLasunción, M. A.
000117453 700__ $$0(orcid)0000-0002-3595-7668$$aRodríguez-Yoldi, M. J.$$uUniversidad de Zaragoza
000117453 700__ $$0(orcid)0000-0002-8100-5596$$aMartínez-Beamonte, R.$$uUniversidad de Zaragoza
000117453 700__ $$0(orcid)0000-0002-8251-8457$$aOsada, J.$$uUniversidad de Zaragoza
000117453 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000117453 7102_ $$11012$$2410$$aUniversidad de Zaragoza$$bDpto. Farmac.Fisiol.y Med.L.F.$$cÁrea Fisiología
000117453 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000117453 7102_ $$11002$$2050$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Biología Celular
000117453 773__ $$g23, 8 (2022), 4172 [16 pp]$$pInt. j. mol. sci.$$tInternational Journal of Molecular Sciences$$x1661-6596
000117453 8564_ $$s3788116$$uhttps://zaguan.unizar.es/record/117453/files/texto_completo.pdf$$yVersión publicada
000117453 8564_ $$s2749774$$uhttps://zaguan.unizar.es/record/117453/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000117453 909CO $$ooai:zaguan.unizar.es:117453$$particulos$$pdriver
000117453 951__ $$a2024-03-18-14:01:50
000117453 980__ $$aARTICLE