117455 20240319081000.0 doi 10.3390/antiox11040733 sideral 128764 ART-2022-128764 eng Paesa, M. Universidad de Zaragoza Valorization of onion waste by obtaining extracts rich in phenolic compounds and feasibility of its therapeutic use on colon cancer 2022 Access copy available to the general public Unrestricted In this study, the total phenolic content, the antioxidant and antiproliferative activities of onion waste extracts were characterized. Some phenolic compounds present in the extracts were also identified and quantified by HPLC-DAD. Additionally, an in-silico analysis was performed to identify the phenolic compounds with the highest intestinal absorption and Caco-2 permeability. The onion extract possessed a high amount of phenolic compounds (177 ± 9 mg/g extract) and had an effective antioxidant capacity measured by ABTS, FRAP and DPPH assays. Regarding the antiproliferative activity, the onion extracts produced cell cycle arrest in the S phase with p53 activation, intrinsic apoptosis (mitochondrial membrane potential modification) and caspase 3 activation. Likewise, onion waste increased intracellular ROS with possible NF-kB activation causing a proteasome down regulation. In addition, the extracts protected the intestine against oxidative stress induced by H2O2 . According to the in-silico analysis, these results could be related to the higher Caco-2 permeability to protocatechuic acid. Therefore, this study provides new insights regarding the potential use of these types of extract as functional ingredients with antioxidant and antiproliferative properties and as medicinal agents in diseases related to oxidative stress, such as cancer. In addition, its valorization would contribute to the circular economy. © 2022 by the authors. Licensee MDPI, Basel, Switzerland. info:eu-repo/grantAgreement/ES/CIBERObn/CB06-03-1012 info:eu-repo/grantAgreement/ES/DGA-FSE/B16-20R info:eu-repo/grantAgreement/EC/H2020/801586/EU/International Doctoral Programme for Talent Attraction to the Campus of International Excellence of the Ebro Valley/IberusTalent This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 801586-IberusTalent info:eu-repo/grantAgreement/ES/MICINN-ISCIIII-FEDER/PID2019-104915RB-I00 info:eu-repo/grantAgreement/EUR/SUDOE/INTERREG/Redvalue-SOE1-PI-E0123 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 7.0 2022 BIOCHEMISTRY & MOLECULAR BIOLOGY 46 / 285 = 0.161 2022 Q1 T1 FOOD SCIENCE & TECHNOLOGY 13 / 142 = 0.092 2022 Q1 T1 CHEMISTRY, MEDICINAL 6 / 60 = 0.1 2022 Q1 T1 1.084 2022 Biochemistry 2022 Q1 Clinical Biochemistry 2022 Q1 Food Science 2022 Q1 Physiology 2022 Q1 Molecular Biology 2022 Q2 Cell Biology 2022 Q2 8.8 2022 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Nogueira, D. P. Velderrain-Rodríguez, G. Esparza, I. Jiménez-Moreno, N. Mendoza, G. Osada, J. Universidad de Zaragoza (orcid)0000-0002-8251-8457 Martin-Belloso, O. Rodríguez-Yoldi, M. J. Universidad de Zaragoza (orcid)0000-0002-3595-7668 Ancín-Azpilicueta, C. 1012 410 Universidad de Zaragoza Dpto. Farmac.Fisiol.y Med.L.F. Área Fisiología 5005 555 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Ingeniería Química 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 11, 4 (2022), 733 [18 pp] Antioxidants Antioxidants 2076-3921 526809 http://zaguan.unizar.es/record/117455/files/texto_completo.pdf Versión publicada 2581129 http://zaguan.unizar.es/record/117455/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:117455 articulos driver 2024-03-18-14:02:00 ARTICLE