000117468 001__ 117468 000117468 005__ 20240319081000.0 000117468 0247_ $$2doi$$a10.3390/biom12040534 000117468 0248_ $$2sideral$$a128771 000117468 037__ $$aART-2022-128771 000117468 041__ $$aeng 000117468 100__ $$aLapuente, J. P. 000117468 245__ $$aCytokine profile and anti-inflammatory activity of a standardized conditioned medium obtained by coculture of monocytes and mesenchymal stromal cells (PRS CK STORM) 000117468 260__ $$c2022 000117468 5060_ $$aAccess copy available to the general public$$fUnrestricted 000117468 5203_ $$aIntercellular communication between monocytes/macrophages and cells involved in tissue regeneration, such as mesenchymal stromal cells (MSCs) and primary tissue cells, is essential for tissue regeneration and recovery of homeostasis. Typically, in the final phase of the inflammation-resolving process, this intercellular communication drives an anti-inflammatory immunomodulatory response. To obtain a safe and effective treatment to counteract the cytokine storm associated with a disproportionate immune response to severe infections, including that associated with COVID-19, by means of naturally balanced immunomodulation, our group has standardized the production under GMP-like conditions of a secretome by coculture of macrophages and MSCs. To characterize this proteome, we determined the expression of molecules related to cellular immune response and tissue regeneration, as well as its possible toxicity and anti-inflammatory potency. The results show a specific molecular pattern of interaction between the two cell types studied, with an anti-inflammatory and regenerative profile. In addition, the secretome is not toxic by itself on human PBMC or on THP-1 monocytes and prevents lipopolysaccharide (LPS)-induced growth effects on those cell types. Finally, PRS CK STORM prevents LPS-induced TNF-A and IL-1B secretion from PBMC and from THP-1 cells at the same level as hydrocortisone, demonstrating its anti-inflammatory potency. © 2022 by the authors. Licensee MDPI, Basel, Switzerland. 000117468 536__ $$9info:eu-repo/grantAgreement/ES/DGA/B31-20R 000117468 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/ 000117468 590__ $$a5.5$$b2022 000117468 592__ $$a1.074$$b2022 000117468 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b70 / 285 = 0.246$$c2022$$dQ1$$eT1 000117468 593__ $$aBiochemistry$$c2022$$dQ1 000117468 593__ $$aMolecular Biology$$c2022$$dQ2 000117468 594__ $$a8.3$$b2022 000117468 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000117468 700__ $$aBlázquez-Martínez, A. 000117468 700__ $$aMarco-Brualla, J. 000117468 700__ $$aGómez, G. 000117468 700__ $$aDesportes, P. 000117468 700__ $$aSanz, J. 000117468 700__ $$aFernández, P. 000117468 700__ $$aGarcía-Gil, M. 000117468 700__ $$aBermejo, F. 000117468 700__ $$aMartín, J. V. S. 000117468 700__ $$aAlgaba, A. 000117468 700__ $$aDe Gregorio, J. C. 000117468 700__ $$aLapuente, D. 000117468 700__ $$aDe Gregorio, A. 000117468 700__ $$aLapuente, B. 000117468 700__ $$ade la Viñas Andrés, M. 000117468 700__ $$0(orcid)0000-0002-5175-8394$$aAnel, A.$$uUniversidad de Zaragoza 000117468 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole. 000117468 773__ $$g12, 4 (2022), 534 [18 pp]$$tBiomolecules$$x2218-273X 000117468 8564_ $$s3033874$$uhttps://zaguan.unizar.es/record/117468/files/texto_completo.pdf$$yVersión publicada 000117468 8564_ $$s2883933$$uhttps://zaguan.unizar.es/record/117468/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000117468 909CO $$ooai:zaguan.unizar.es:117468$$particulos$$pdriver 000117468 951__ $$a2024-03-18-14:05:28 000117468 980__ $$aARTICLE