000117471 001__ 117471
000117471 005__ 20240319081000.0
000117471 0247_ $$2doi$$a10.1039/d1nr08513a
000117471 0248_ $$2sideral$$a128777
000117471 037__ $$aART-2022-128777
000117471 041__ $$aeng
000117471 100__ $$aGarcés, V.
000117471 245__ $$aMagneto-optical hyperthermia agents based on probiotic bacteria loaded with magnetic and gold nanoparticles
000117471 260__ $$c2022
000117471 5060_ $$aAccess copy available to the general public$$fUnrestricted
000117471 5203_ $$aProbiotic bacteria were used as carriers of metallic nanoparticles to develop innovative oral agents for hyperthermia cancer therapy. Two synthetic strategies were used to produce the different therapeutic agents. First, the probiotic bacterium Lactobacillus fermentum was simultaneously loaded with magnetic (MNPs) and gold nanoparticles (AuNPs) of different morphologies to produce AuNP + MNP-bacteria systems with both types of nanoparticles arranged in the same layer of bacterial exopolysaccharides (EPS). In the second approach, the probiotic was first loaded with AuNP to form AuNP-bacteria and subsequently loaded with MNP-EPS to yield AuNP-bacteria-EPS-MNP with the MNP and AuNP arranged in two different EPS layers. This second strategy has never been reported and exploits the presence of EPS-EPS recognition which allows the layer-by-layer formation of structures on the bacteria external wall. The AuNP + MNP-bacteria and AuNP-bacteria-EPS-MNP samples were characterized by scanning (SEM) and transmission electron microscopy (TEM), and UV-vis spectroscopy. The potential of these two heterobimetallic systems as magnetic hyperthermia or photothermal therapy agents was assessed, validating their capacity to produce heat either during exposure to an alternating magnetic field or near-infrared laser light. The probiotic Lactobacillus fermentum has already been proposed as an oral drug carrier, able to overcome the stomach medium and deliver drugs to the intestines, and it is actually marketed as an oral supplement to reinforce the gut microbiota, thus, our results open the way for the development of novel therapeutic strategies using these new heterobimetallic AuNP/MNP-bacteria systems in the frame of gastric diseases, using them, for example, as oral agents for cancer treatment with magnetic hyperthermia and photothermal therapy. © 2022 The Royal Society of Chemistry
000117471 536__ $$9info:eu-repo/grantAgreement/EC/H2020/823717/EU/Enabling Science and Technology through European Electron Microscopy/ESTEEM3$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 823717-ESTEEM3$$9info:eu-repo/grantAgreement/ES/MICINN/PGC2018-096016-B-I00$$9info:eu-repo/grantAgreement/ES/MICINN/PID2019-111461GB-I00$$9info:eu-repo/grantAgreement/ES/MICINN/RYC-2016-21042
000117471 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc$$uhttp://creativecommons.org/licenses/by-nc/3.0/es/
000117471 590__ $$a6.7$$b2022
000117471 592__ $$a1.62$$b2022
000117471 591__ $$aMATERIALS SCIENCE, MULTIDISCIPLINARY$$b83 / 343 = 0.242$$c2022$$dQ1$$eT1
000117471 593__ $$aNanoscience and Nanotechnology$$c2022$$dQ1
000117471 591__ $$aPHYSICS, APPLIED$$b27 / 160 = 0.169$$c2022$$dQ1$$eT1
000117471 593__ $$aMaterials Science (miscellaneous)$$c2022$$dQ1
000117471 591__ $$aCHEMISTRY, MULTIDISCIPLINARY$$b43 / 178 = 0.242$$c2022$$dQ1$$eT1
000117471 591__ $$aNANOSCIENCE & NANOTECHNOLOGY$$b37 / 107 = 0.346$$c2022$$dQ2$$eT2
000117471 594__ $$a13.6$$b2022
000117471 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000117471 700__ $$aGonzález, A.
000117471 700__ $$aGálvez, N.
000117471 700__ $$aDelgado-López, J. M.
000117471 700__ $$aCalvino, J. J.
000117471 700__ $$aTrasobares, S.
000117471 700__ $$0(orcid)0000-0002-0970-1917$$aFernández-Afonso, Y.
000117471 700__ $$0(orcid)0000-0003-2366-3598$$aGutiérrez, L.$$uUniversidad de Zaragoza
000117471 700__ $$aDominguez-Vera, J.
000117471 7102_ $$12009$$2750$$aUniversidad de Zaragoza$$bDpto. Química Analítica$$cÁrea Química Analítica
000117471 773__ $$g14, 15 (2022), 5716-5724$$pNanoscale$$tNanoscale$$x2040-3364
000117471 8564_ $$s2483415$$uhttps://zaguan.unizar.es/record/117471/files/texto_completo.pdf$$yVersión publicada
000117471 8564_ $$s2611630$$uhttps://zaguan.unizar.es/record/117471/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000117471 909CO $$ooai:zaguan.unizar.es:117471$$particulos$$pdriver
000117471 951__ $$a2024-03-18-14:06:03
000117471 980__ $$aARTICLE