000117472 001__ 117472
000117472 005__ 20240319081000.0
000117472 0247_ $$2doi$$a10.1039/d1nr08223j
000117472 0248_ $$2sideral$$a128778
000117472 037__ $$aART-2022-128778
000117472 041__ $$aeng
000117472 100__ $$0(orcid)0000-0002-0203-5843$$aSoria-Carrera, H.
000117472 245__ $$aPolyoxometalate-polypeptide nanoassemblies as peroxidase surrogates with antibiofilm properties
000117472 260__ $$c2022
000117472 5060_ $$aAccess copy available to the general public$$fUnrestricted
000117472 5203_ $$aDeveloping artificial metalloenzymes that possess a superior performance to their natural counterparts is an attractive concept. Polyoxometalates (POMs) are a class of anionic molecular metal-oxides with excellent redox properties and bioactivity. We have recently introduced “POMlymers” - covalently conjugated POM-peptide hybrid materials - where the polypeptide chain is obtained through a ring-opening polymerisation (ROP) of a-amino acid N-carboxyanhydrides (NCA) on an inorganic POM scaffold. Attracted by the idea of preparing artificial metalloenzymes, here we report the supramolecular self-assembly of POMlymer hybrids into nanoparticles where an optimal environment for catalysis is created. Our results demonstrate that the self-assembly of covalent POMlymers, enhances the peroxidase-like activity of the parent POM anion whereas, in contrast, the catalytic activity for nanoparticles obtained by ionic self-assembly of the same peptide and POM components practically disappears. Furthermore, POMlymer nanoparticles also present antimicrobial and antibiofilm activity against the skin bacterium Staphylococcus epidermidis; whereas, ionic POM-peptide hybrids significantly increase biofilm production and endogenous production of reactive oxygen species. In summary, we present the self-assembly of POMlymer hybrids into nanoparticles and a combination of peroxidase activity and microbiology assays that show that the POM-peptide covalent bond is essential for the stability of the self-assembled nanoparticles and therefore for their catalytic and biological activity. © 2022 The Royal Society of Chemistry.
000117472 536__ $$9info:eu-repo/grantAgreement/ES/CSIC/i-Link+2019-LINK20270$$9info:eu-repo/grantAgreement/ES/DGA/E15-20R$$9info:eu-repo/grantAgreement/EC/H2020/845427/EU/Peptide-functionalized POMs as biofilm disruption agents: searching for synergy in bactericidal materials/PePiPOM$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 845427-PePiPOM$$9info:eu-repo/grantAgreement/ES/MEC/FPU2016-02456$$9info:eu-repo/grantAgreement/ES/MICINN/PID2019-109333RB-I00
000117472 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000117472 590__ $$a6.7$$b2022
000117472 592__ $$a1.62$$b2022
000117472 591__ $$aMATERIALS SCIENCE, MULTIDISCIPLINARY$$b83 / 343 = 0.242$$c2022$$dQ1$$eT1
000117472 593__ $$aNanoscience and Nanotechnology$$c2022$$dQ1
000117472 591__ $$aPHYSICS, APPLIED$$b27 / 160 = 0.169$$c2022$$dQ1$$eT1
000117472 593__ $$aMaterials Science (miscellaneous)$$c2022$$dQ1
000117472 591__ $$aCHEMISTRY, MULTIDISCIPLINARY$$b43 / 178 = 0.242$$c2022$$dQ1$$eT1
000117472 591__ $$aNANOSCIENCE & NANOTECHNOLOGY$$b37 / 107 = 0.346$$c2022$$dQ2$$eT2
000117472 594__ $$a13.6$$b2022
000117472 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000117472 700__ $$0(orcid)0000-0002-3830-7847$$aAtrián-Blasco, E.
000117472 700__ $$ade la Fuente, J. M.
000117472 700__ $$0(orcid)0000-0003-4848-414X$$aMitchell, S. G.
000117472 700__ $$0(orcid)0000-0003-0702-8260$$aMartín-Rapún, R.$$uUniversidad de Zaragoza
000117472 7102_ $$12013$$2765$$aUniversidad de Zaragoza$$bDpto. Química Orgánica$$cÁrea Química Orgánica
000117472 773__ $$g14, 16 (2022), 5999-6006$$pNanoscale$$tNanoscale$$x2040-3364
000117472 8564_ $$s2847688$$uhttps://zaguan.unizar.es/record/117472/files/texto_completo.pdf$$yVersión publicada
000117472 8564_ $$s1468738$$uhttps://zaguan.unizar.es/record/117472/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000117472 909CO $$ooai:zaguan.unizar.es:117472$$particulos$$pdriver
000117472 951__ $$a2024-03-18-14:06:16
000117472 980__ $$aARTICLE