117472 20240319081000.0 doi 10.1039/d1nr08223j sideral 128778 ART-2022-128778 eng Soria-Carrera, H. (orcid)0000-0002-0203-5843 Polyoxometalate-polypeptide nanoassemblies as peroxidase surrogates with antibiofilm properties 2022 Access copy available to the general public Unrestricted Developing artificial metalloenzymes that possess a superior performance to their natural counterparts is an attractive concept. Polyoxometalates (POMs) are a class of anionic molecular metal-oxides with excellent redox properties and bioactivity. We have recently introduced “POMlymers” - covalently conjugated POM-peptide hybrid materials - where the polypeptide chain is obtained through a ring-opening polymerisation (ROP) of a-amino acid N-carboxyanhydrides (NCA) on an inorganic POM scaffold. Attracted by the idea of preparing artificial metalloenzymes, here we report the supramolecular self-assembly of POMlymer hybrids into nanoparticles where an optimal environment for catalysis is created. Our results demonstrate that the self-assembly of covalent POMlymers, enhances the peroxidase-like activity of the parent POM anion whereas, in contrast, the catalytic activity for nanoparticles obtained by ionic self-assembly of the same peptide and POM components practically disappears. Furthermore, POMlymer nanoparticles also present antimicrobial and antibiofilm activity against the skin bacterium Staphylococcus epidermidis; whereas, ionic POM-peptide hybrids significantly increase biofilm production and endogenous production of reactive oxygen species. In summary, we present the self-assembly of POMlymer hybrids into nanoparticles and a combination of peroxidase activity and microbiology assays that show that the POM-peptide covalent bond is essential for the stability of the self-assembled nanoparticles and therefore for their catalytic and biological activity. © 2022 The Royal Society of Chemistry. info:eu-repo/grantAgreement/ES/CSIC/i-Link+2019-LINK20270 info:eu-repo/grantAgreement/ES/DGA/E15-20R info:eu-repo/grantAgreement/EC/H2020/845427/EU/Peptide-functionalized POMs as biofilm disruption agents: searching for synergy in bactericidal materials/PePiPOM This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 845427-PePiPOM info:eu-repo/grantAgreement/ES/MEC/FPU2016-02456 info:eu-repo/grantAgreement/ES/MICINN/PID2019-109333RB-I00 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 6.7 2022 MATERIALS SCIENCE, MULTIDISCIPLINARY 83 / 343 = 0.242 2022 Q1 T1 PHYSICS, APPLIED 27 / 160 = 0.169 2022 Q1 T1 CHEMISTRY, MULTIDISCIPLINARY 43 / 178 = 0.242 2022 Q1 T1 NANOSCIENCE & NANOTECHNOLOGY 37 / 107 = 0.346 2022 Q2 T2 1.62 2022 Nanoscience and Nanotechnology 2022 Q1 Materials Science (miscellaneous) 2022 Q1 13.6 2022 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Atrián-Blasco, E. (orcid)0000-0002-3830-7847 de la Fuente, J. M. Mitchell, S. G. (orcid)0000-0003-4848-414X Martín-Rapún, R. Universidad de Zaragoza (orcid)0000-0003-0702-8260 2013 765 Universidad de Zaragoza Dpto. Química Orgánica Área Química Orgánica 14, 16 (2022), 5999-6006 Nanoscale Nanoscale 2040-3364 2847688 http://zaguan.unizar.es/record/117472/files/texto_completo.pdf Versión publicada 1468738 http://zaguan.unizar.es/record/117472/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:117472 articulos driver 2024-03-18-14:06:16 ARTICLE