000117477 001__ 117477 000117477 005__ 20240319081001.0 000117477 0247_ $$2doi$$a10.3389/fpls.2022.824781 000117477 0248_ $$2sideral$$a128783 000117477 037__ $$aART-2022-128783 000117477 041__ $$aeng 000117477 100__ $$aMorcillo, M. 000117477 245__ $$aEffect of methyl jasmonate in gene expression, and in hormonal and phenolic profiles of holm oak embryogenic lines before and after infection with Phytophthora Cinnamomi 000117477 260__ $$c2022 000117477 5060_ $$aAccess copy available to the general public$$fUnrestricted 000117477 5203_ $$aThe dieback syndrome affecting Quercus ilex and other oak species impels the search for tolerant plant genotypes, as well as methods of plant immunization against such infections. Elicitation treatments can be an effective strategy to activate plant defense response and embryogenic lines represent a promising tool to generate new tolerant genotypes and also to study early markers involved in defense response. The aim of the presented work was to investigate changes in gene expression, and in hormonal and phenolic profiles induced in three holm oak embryogenic lines (ELs) elicited with methyl jasmonate (MeJA) before and after infection with the oomycete Phytophthora cinnamomi, which is the main biotic agent involved in this pathogenic process. The three ELs, derived from three genotypes, showed different basal profiles in all tested parameters, noting that the VA5 naïve genotype from a scape tree was characterized by a basal higher expression in NADPH-dependent cinnamyl alcohol dehydrogenase (CAD) and chalcone synthase (CHS) genes and also by higher caffeic acid content. Our work also identifies changes triggered by MeJA elicitation in holm oak embryogenic lines, such as increases in ABA and JA contents, as well as in levels of most of the determined phenolic compounds, especially in caffeic acid in Q8 and E00 ELs, but not in their biosynthesis genes. Irrespective of the EL, the response to oomycete infection in holm oak elicited plant material was characterized by a further increase in JA. Since JA and phenols have been described as a part of the Q. ilex defense response against P. cinnamomi, we propose that MeJA may act as an induced resistance (IR) stimulus and that in our embryogenic material induced both direct (detected prior to any challenge) and primed (detected after subsequent challenge) defense responses. Copyright © 2022 Morcillo, Sales, Corredoira, Martínez, Segura and Arrillaga. 000117477 536__ $$9info:eu-repo/grantAgreement/ES/MICINN/BES-2014-069171$$9info:eu-repo/grantAgreement/ES/MICINN-FEDER/AGL2013-47400-C4-04-R$$9info:eu-repo/grantAgreement/ES/MICINN-FEDER/AGL2016-76143-C4-01-R$$9info:eu-repo/grantAgreement/ES/MICINN-FEDER/AGL2016-76143-C4-04-R$$9info:eu-repo/grantAgreement/ES/MICINN/PID2020-112627RB 000117477 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/ 000117477 590__ $$a5.6$$b2022 000117477 592__ $$a1.231$$b2022 000117477 591__ $$aPLANT SCIENCES$$b27 / 239 = 0.113$$c2022$$dQ1$$eT1 000117477 593__ $$aPlant Science$$c2022$$dQ1 000117477 594__ $$a7.1$$b2022 000117477 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000117477 700__ $$0(orcid)0000-0003-0582-5418$$aSales, E.$$uUniversidad de Zaragoza 000117477 700__ $$aCorredoira, E. 000117477 700__ $$aMartínez, M. T. 000117477 700__ $$aSegura, J. 000117477 700__ $$aArrillaga, I. 000117477 7102_ $$15011$$2705$$aUniversidad de Zaragoza$$bDpto. CC.Agrar.y Medio Natural$$cÁrea Producción Vegetal 000117477 773__ $$g13 (2022), 824781 [13 pp]$$pFront. plant sci.$$tFrontiers in Plant Science$$x1664-462X 000117477 8564_ $$s1365056$$uhttps://zaguan.unizar.es/record/117477/files/texto_completo.pdf$$yVersión publicada 000117477 8564_ $$s2409348$$uhttps://zaguan.unizar.es/record/117477/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000117477 909CO $$ooai:zaguan.unizar.es:117477$$particulos$$pdriver 000117477 951__ $$a2024-03-18-14:10:12 000117477 980__ $$aARTICLE