117603 20230519145514.0 doi 10.1093/jac/dkab208 sideral 124568 ART-2021-124568 eng Mama, O.M. Penicillin susceptibility among invasive MSSA infections: a multicentre study in 16 Spanish hospitals 2021 Access copy available to the general public Unrestricted Objectives: To determine the prevalence of penicillin susceptibility among MSSA causing bloodstream infections (BSIs) in 16 Spanish hospitals and to characterize the penicillin-susceptible MSSA (MSSA-PENS) isolates. Methods: A total of 1011 Staphylococcus aureus isolates were collected from blood cultures in 16 Spanish hospitals during 2018–19 (6–12 months) and their susceptibility to 18 antimicrobials was determined. The MSSA-PENS isolates were selected and examined by PCR to determine the presence of the blaZ gene, other resistance genes and the genes lukF/lukS-PV, eta, etb and tst. The immune evasion cluster (IEC) type was also analysed. All the MSSA-PENS isolates were submitted to S. aureus protein A (spa) typing and the clonal complexes (CCs) were assigned according to their spa type. Results: The prevalence of MSSA was 74.6% (754/1011) and 14.9% (151/1011) were MSSA-PENS-blaZnegative. MSSA-PENS-blaZnegative isolates (n = 151) were ascribed to 88 spa types and 11 CCs. The most frequent CCs were CC5 (35/151) and CC398 (25/151), with t002-CC5 and t571-CC398 being the most common lineages. Pan-susceptibility was identified in 117 of the 151 MSSA-PENS-blaZnegative isolates (77.5%). In the remaining isolates, erythromycin and clindamycin resistance was the most frequent resistance found, although tobramycin, ciprofloxacin, fusidic acid, mupirocin and/or tetracycline resistance was also detected. Thirty-eight MSSA-PENS-blaZnegative isolates were IEC negative and four isolates were Panton–Valentine leucocidin (‘PVL’) positive. Conclusions: A high penicillin susceptibility rate was detected among MSSA, opening therapeutic opportunities for BSIs. The emergence of new successful MSSA-PENS clones could be responsible for these data. The detection among MSSA-PENS-blaZnegative isolates of the clonal lineage CC398 or the absence of an IEC raises questions about their possible animal origin, requiring further analysis. info:eu-repo/grantAgreement/ES/AEI-FEDER-ICS/SAF2016–76571-R info:eu-repo/grantAgreement/ES/AEI/PID2019-106158RB-I00 info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 5.758 2021 PHARMACOLOGY & PHARMACY 54 / 279 = 0.194 2021 Q1 T1 INFECTIOUS DISEASES 32 / 96 = 0.333 2021 Q2 T2 MICROBIOLOGY 39 / 138 = 0.283 2021 Q2 T1 1.495 2021 Infectious Diseases 2021 Q1 Pharmacology (medical) 2021 Q1 Microbiology (medical) 2021 Q1 9.4 2021 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Aspiroz, C. Lozano, C. Ruiz-Ripa, L. Azcona, J.M. Seral, C. Universidad de Zaragoza (orcid)0000-0002-9742-1463 Cercenado, E. López-Cerero, L. Palacián, P. Belles-Belles, A. Berdonces, P. Siller, M. Aguirre-Quiñonero, A. Zarazaga, M. Torres, C Castillo, J. Universidad de Zaragoza (orcid)0000-0002-2519-701X Rezusta, A. Universidad de Zaragoza (orcid)0000-0001-7294-245X Calleja, A.I. on behalf of the Spanish Study Group of Clinical S. aureus CC398 1011 630 Universidad de Zaragoza Dpto. Microb.Ped.Radio.Sal.Pú. Área Microbiología 76, 10 (2021), 2519–2527 J. antimicrob. chemother. The Journal of antimicrobial chemotherapy 0305-7453 426113 http://zaguan.unizar.es/record/117603/files/texto_completo.pdf Postprint 2797067 http://zaguan.unizar.es/record/117603/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:117603 articulos driver 2023-05-18-15:14:43 ARTICLE