000117629 001__ 117629
000117629 005__ 20240319081008.0
000117629 0247_ $$2doi$$a10.3390/jof8040361
000117629 0248_ $$2sideral$$a129191
000117629 037__ $$aART-2022-129191
000117629 041__ $$aeng
000117629 100__ $$aRedrado, S.
000117629 245__ $$aIntegration of In silico and in vitro analysis of gliotoxin production reveals a narrow range of producing fungal species
000117629 260__ $$c2022
000117629 5060_ $$aAccess copy available to the general public$$fUnrestricted
000117629 5203_ $$aGliotoxin is a fungal secondary metabolite with impact on health and agriculture since it might act as virulence factor and contaminate human and animal food. Homologous gliotoxin (GT) gene clusters are spread across a number of fungal species although if they produce GT or other related epipolythiodioxopiperazines (ETPs) remains obscure. Using bioinformatic tools, we have identified homologous gli gene clusters similar to the A. fumigatus GT gene cluster in several fungal species. In silico study led to in vitro confirmation of GT and Bisdethiobis(methylthio)gliotoxin (bmGT) production in fungal strain cultures by HPLC detection. Despite we selected most similar homologous gli gene cluster in 20 different species, GT and bmGT were only detected in section Fumigati species and in a Trichoderma virens Q strain. Our results suggest that in silico gli homology analyses in different fungal strains to predict GT production might be only informative when accompanied by analysis about mycotoxin production in cell cultures.
000117629 536__ $$9info:eu-repo/grantAgreement/ES/AEI/PID2020-113963RB-I00$$9info:eu-repo/grantAgreement/ES/AEI/PTA2019-016739-I$$9info:eu-repo/grantAgreement/ES/DGA/B29-20R$$9info:eu-repo/grantAgreement/ES/MINECO/IJC-2019-039192-I$$9info:eu-repo/grantAgreement/ES/MINECO/SAF2017-83120-C2-1-R
000117629 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000117629 590__ $$a4.7$$b2022
000117629 592__ $$a0.871$$b2022
000117629 591__ $$aMYCOLOGY$$b9 / 30 = 0.3$$c2022$$dQ2$$eT1
000117629 593__ $$aEcology, Evolution, Behavior and Systematics$$c2022$$dQ1
000117629 591__ $$aMICROBIOLOGY$$b45 / 135 = 0.333$$c2022$$dQ2$$eT2
000117629 593__ $$aPlant Science$$c2022$$dQ1
000117629 593__ $$aMicrobiology (medical)$$c2022$$dQ2
000117629 594__ $$a4.9$$b2022
000117629 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000117629 700__ $$aEsteban, P.
000117629 700__ $$aDomingo, M. P.
000117629 700__ $$aLópez, C.$$uUniversidad de Zaragoza
000117629 700__ $$0(orcid)0000-0001-7294-245X$$aRezusta, A.$$uUniversidad de Zaragoza
000117629 700__ $$aRamirez-Labrada, A.
000117629 700__ $$aArias, M.
000117629 700__ $$0(orcid)0000-0003-0154-0730$$aPardo, J.$$uUniversidad de Zaragoza
000117629 700__ $$aGalvez, E. M.
000117629 7102_ $$11006$$2255$$aUniversidad de Zaragoza$$bDpto. Fisiatría y Enfermería$$cÁrea Enfermería
000117629 7102_ $$11011$$2630$$aUniversidad de Zaragoza$$bDpto. Microb.Ped.Radio.Sal.Pú.$$cÁrea Microbiología
000117629 7102_ $$11011$$2566$$aUniversidad de Zaragoza$$bDpto. Microb.Ped.Radio.Sal.Pú.$$cÁrea Inmunología
000117629 773__ $$g8, 4 (2022), 361 [19 pp.]$$pJ. Fungi$$tJournal of Fungi$$x2309-608X
000117629 8564_ $$s3507646$$uhttps://zaguan.unizar.es/record/117629/files/texto_completo.pdf$$yVersión publicada
000117629 8564_ $$s2609980$$uhttps://zaguan.unizar.es/record/117629/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000117629 909CO $$ooai:zaguan.unizar.es:117629$$particulos$$pdriver
000117629 951__ $$a2024-03-18-14:52:19
000117629 980__ $$aARTICLE