117629 20240319081008.0 doi 10.3390/jof8040361 sideral 129191 ART-2022-129191 eng Redrado, S. Integration of In silico and in vitro analysis of gliotoxin production reveals a narrow range of producing fungal species 2022 Access copy available to the general public Unrestricted Gliotoxin is a fungal secondary metabolite with impact on health and agriculture since it might act as virulence factor and contaminate human and animal food. Homologous gliotoxin (GT) gene clusters are spread across a number of fungal species although if they produce GT or other related epipolythiodioxopiperazines (ETPs) remains obscure. Using bioinformatic tools, we have identified homologous gli gene clusters similar to the A. fumigatus GT gene cluster in several fungal species. In silico study led to in vitro confirmation of GT and Bisdethiobis(methylthio)gliotoxin (bmGT) production in fungal strain cultures by HPLC detection. Despite we selected most similar homologous gli gene cluster in 20 different species, GT and bmGT were only detected in section Fumigati species and in a Trichoderma virens Q strain. Our results suggest that in silico gli homology analyses in different fungal strains to predict GT production might be only informative when accompanied by analysis about mycotoxin production in cell cultures. info:eu-repo/grantAgreement/ES/AEI/PID2020-113963RB-I00 info:eu-repo/grantAgreement/ES/AEI/PTA2019-016739-I info:eu-repo/grantAgreement/ES/DGA/B29-20R info:eu-repo/grantAgreement/ES/MINECO/IJC-2019-039192-I info:eu-repo/grantAgreement/ES/MINECO/SAF2017-83120-C2-1-R info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 4.7 2022 MYCOLOGY 9 / 30 = 0.3 2022 Q2 T1 MICROBIOLOGY 45 / 135 = 0.333 2022 Q2 T2 0.871 2022 Ecology, Evolution, Behavior and Systematics 2022 Q1 Plant Science 2022 Q1 Microbiology (medical) 2022 Q2 4.9 2022 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Esteban, P. Domingo, M. P. López, C. Universidad de Zaragoza Rezusta, A. Universidad de Zaragoza (orcid)0000-0001-7294-245X Ramirez-Labrada, A. Arias, M. Pardo, J. Universidad de Zaragoza (orcid)0000-0003-0154-0730 Galvez, E. M. 1006 255 Universidad de Zaragoza Dpto. Fisiatría y Enfermería Área Enfermería 1011 630 Universidad de Zaragoza Dpto. Microb.Ped.Radio.Sal.Pú. Área Microbiología 1011 566 Universidad de Zaragoza Dpto. Microb.Ped.Radio.Sal.Pú. Área Inmunología 8, 4 (2022), 361 [19 pp.] J. Fungi Journal of Fungi 2309-608X 3507646 http://zaguan.unizar.es/record/117629/files/texto_completo.pdf Versión publicada 2609980 http://zaguan.unizar.es/record/117629/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:117629 articulos driver 2024-03-18-14:52:19 ARTICLE