000117632 001__ 117632
000117632 005__ 20240319081010.0
000117632 0247_ $$2doi$$a10.1111/rda.14137
000117632 0248_ $$2sideral$$a129226
000117632 037__ $$aART-2022-129226
000117632 041__ $$aeng
000117632 100__ $$aFernandez-Alegre, Estela
000117632 245__ $$aMelatonin affects red deer spermatozoa motility and physiology in capacitating and non-capacitating conditions
000117632 260__ $$c2022
000117632 5060_ $$aAccess copy available to the general public$$fUnrestricted
000117632 5203_ $$aMelatonin affects sperm physiology, possibly through membrane receptors. Effects were tested at low concentrations (1 pM, 100 pM, 10 nM and 1 mu M) in red deer epididymal spermatozoa as a model for high-seasonality species. Samples were incubated with melatonin as uncapacitated or capacitating conditions (heparin) and evaluated for motility and physiology (flow cytometry). Most effects occurred at low concentrations (nM-pM), mainly protecting from apoptosis and maintaining acrosomal integrity, suggesting a role for membrane receptors rather than a direct antioxidant effect. Intracellular calcium was not affected, differing from other studies and perhaps because of the epididymal origin. This study supports the relevance of melatonin on sperm physiology and could contribute to the application of reproductive technologies in wild ruminants.
000117632 536__ $$9info:eu-repo/grantAgreement/ES/MINECO-FEDER/AGL2013-43328-P
000117632 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000117632 590__ $$a1.7$$b2022
000117632 592__ $$a0.465$$b2022
000117632 591__ $$aVETERINARY SCIENCES$$b55 / 144 = 0.382$$c2022$$dQ2$$eT2
000117632 591__ $$aAGRICULTURE, DAIRY & ANIMAL SCIENCE$$b33 / 62 = 0.532$$c2022$$dQ3$$eT2
000117632 591__ $$aREPRODUCTIVE BIOLOGY$$b29 / 31 = 0.935$$c2022$$dQ4$$eT3
000117632 593__ $$aAnimal Science and Zoology$$c2022$$dQ2
000117632 593__ $$aEndocrinology$$c2022$$dQ3
000117632 593__ $$aBiotechnology$$c2022$$dQ3
000117632 594__ $$a3.2$$b2022
000117632 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000117632 700__ $$aLacalle, Estibaliz
000117632 700__ $$aSoriano-Ubeda, Cristina
000117632 700__ $$aCarlos Dominguez, Juan
000117632 700__ $$0(orcid)0000-0003-1997-4262$$aCasao, Adriana$$uUniversidad de Zaragoza
000117632 700__ $$aMartinez-Pastor, Felipe
000117632 7102_ $$11002$$2819$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Zoología
000117632 773__ $$g57, 55 (2022), 82-85$$pReprod. domest. anim.$$tReproduction in Domestic Animals$$x0936-6768
000117632 8564_ $$s368739$$uhttps://zaguan.unizar.es/record/117632/files/texto_completo.pdf$$yVersión publicada
000117632 8564_ $$s2263724$$uhttps://zaguan.unizar.es/record/117632/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000117632 909CO $$ooai:zaguan.unizar.es:117632$$particulos$$pdriver
000117632 951__ $$a2024-03-18-15:00:47
000117632 980__ $$aARTICLE