117632 20240319081010.0 doi 10.1111/rda.14137 sideral 129226 ART-2022-129226 eng Fernandez-Alegre, Estela Melatonin affects red deer spermatozoa motility and physiology in capacitating and non-capacitating conditions 2022 Access copy available to the general public Unrestricted Melatonin affects sperm physiology, possibly through membrane receptors. Effects were tested at low concentrations (1 pM, 100 pM, 10 nM and 1 mu M) in red deer epididymal spermatozoa as a model for high-seasonality species. Samples were incubated with melatonin as uncapacitated or capacitating conditions (heparin) and evaluated for motility and physiology (flow cytometry). Most effects occurred at low concentrations (nM-pM), mainly protecting from apoptosis and maintaining acrosomal integrity, suggesting a role for membrane receptors rather than a direct antioxidant effect. Intracellular calcium was not affected, differing from other studies and perhaps because of the epididymal origin. This study supports the relevance of melatonin on sperm physiology and could contribute to the application of reproductive technologies in wild ruminants. info:eu-repo/grantAgreement/ES/MINECO-FEDER/AGL2013-43328-P info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 1.7 2022 VETERINARY SCIENCES 55 / 144 = 0.382 2022 Q2 T2 AGRICULTURE, DAIRY & ANIMAL SCIENCE 33 / 62 = 0.532 2022 Q3 T2 REPRODUCTIVE BIOLOGY 29 / 31 = 0.935 2022 Q4 T3 0.465 2022 Animal Science and Zoology 2022 Q2 Endocrinology 2022 Q3 Biotechnology 2022 Q3 3.2 2022 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Lacalle, Estibaliz Soriano-Ubeda, Cristina Carlos Dominguez, Juan Casao, Adriana Universidad de Zaragoza (orcid)0000-0003-1997-4262 Martinez-Pastor, Felipe 1002 819 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Zoología 57, 55 (2022), 82-85 Reprod. domest. anim. Reproduction in Domestic Animals 0936-6768 368739 http://zaguan.unizar.es/record/117632/files/texto_completo.pdf Versión publicada 2263724 http://zaguan.unizar.es/record/117632/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:117632 articulos driver 2024-03-18-15:00:47 ARTICLE