117640 20240319081014.0 doi 10.1016/j.idairyj.2022.105428 sideral 129069 ART-2022-129069 eng Abad, Inés Universidad de Zaragoza Protective effect of bovine lactoferrin against Cronobacter sakazakii in human intestinal Caco-2/TC7 cells 2022 Access copy available to the general public Unrestricted Milk is a source of bioactive proteins with defensive properties of great value for protecting the newborn. The activity of bovine milk lactoferrin (LF) was investigated as an antibacterial agent in the internalisation of the emergent pathogen Cronobacter sakazakii into Caco-2/TC7 cells, a model of human intestinal epithelium. The effect of LF on oxidative stress and expression of Toll-like receptors (TLR) was also investigated. LF exerted a clear antibacterial activity against C. sakazakii, as well as an inhibitory effect on C. sakazakii adhesion and internalisation into Caco-2/TC7 cells. Incubation with C. sakazakii induced an increase in oxidative stress on the lipid fraction of Caco-2/TC7 cells, which was reversed by LF. Additionally, LF altered the expression of TLR, with a clear decrease in the expression levels of TLR4 at 24 h of incubation. These results suggest that LF has very interesting properties as a potential ingredient for functional foods. info:eu-repo/grantAgreement/ES/DGA-FEDER/A02-20R info:eu-repo/grantAgreement/ES/MINECO/AGL2017-82987 info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 3.1 2022 FOOD SCIENCE & TECHNOLOGY 69 / 142 = 0.486 2022 Q2 T2 0.726 2022 Food Science 2022 Q1 Applied Microbiology and Biotechnology 2022 Q2 6.1 2022 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Sangüesa, Andrea Ubieto, María Carramiñana, Juan J. Universidad de Zaragoza (orcid)0000-0002-3320-9295 Pérez, María D. Universidad de Zaragoza (orcid)0000-0003-2555-8425 Buey, Berta Universidad de Zaragoza (orcid)0000-0002-8467-0356 Mesonero, José E. Universidad de Zaragoza (orcid)0000-0003-4758-3998 Grasa, Laura Universidad de Zaragoza (orcid)0000-0002-5306-9365 Sánchez, Lourdes Universidad de Zaragoza (orcid)0000-0001-5964-823X 2008 640 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Nutrición Bromatología 1012 410 Universidad de Zaragoza Dpto. Farmac.Fisiol.y Med.L.F. Área Fisiología 2008 780 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Tecnología de Alimentos 133 (2022), 105428 [12 pp.] Int. dairy j. INTERNATIONAL DAIRY JOURNAL 0958-6946 1468000 http://zaguan.unizar.es/record/117640/files/texto_completo.pdf Versión publicada 2706518 http://zaguan.unizar.es/record/117640/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:117640 articulos driver 2024-03-18-15:29:06 ARTICLE