000117646 001__ 117646
000117646 005__ 20240319081015.0
000117646 0247_ $$2doi$$a10.1039/d2bm00365a
000117646 0248_ $$2sideral$$a128969
000117646 037__ $$aART-2022-128969
000117646 041__ $$aeng
000117646 100__ $$0(orcid)0000-0003-0063-9456$$aSan Anselmo, Maria
000117646 245__ $$aDendron-functionalised hyperbranched bis-MPA polyesters as efficient non-viral vectors for gene therapy in different cell lines
000117646 260__ $$c2022
000117646 5060_ $$aAccess copy available to the general public$$fUnrestricted
000117646 5203_ $$aGene therapy has become a relevant tool in the biomedical field to treat or even prevent some diseases. The effective delivery of genetic material into the cell remains a crucial step to succeed in this purpose. In the search for efficient non-viral vectors, a series of amino-terminated dendronized hyperbranched polymers (DHPs) of different generations based either on bis-MPA or bis-GMPA have been designed. All of them have demonstrated an accurate ability to complex two types of genetic materials, a plasmid DNA and a siGFP, yielding dendriplexes. Moreover, some of them have proved to be able to deliver the genetic material inside the cells, resulting in the effective accomplishment of the desired genetic modification and improving the activity of some commercial transfection reagents. Different cell lines, including cancer and mesenchymal stem cells, have been studied here to evaluate the ability of DHPs as vectors for transfection. Treatments based on mesenchymal stem cells are gaining importance due to their pluripotency. Thus, it is of special relevance to introduce a genetic modification into a mesenchymal cell line as it allows it to act over a wide spectrum of tissues after inducing cellular differentiation.
000117646 536__ $$9info:eu-repo/grantAgreement/ES/DGA-FSE/E47-20R$$9info:eu-repo/grantAgreement/ES/MCIU/BES-2016-078774$$9info:eu-repo/grantAgreement/ES/MCIU/PGC2018-097583-B-I00
000117646 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000117646 590__ $$a6.6$$b2022
000117646 592__ $$a1.171$$b2022
000117646 591__ $$aMATERIALS SCIENCE, BIOMATERIALS$$b12 / 45 = 0.267$$c2022$$dQ2$$eT1
000117646 593__ $$aMaterials Science (miscellaneous)$$c2022$$dQ1
000117646 593__ $$aBiomedical Engineering$$c2022$$dQ1
000117646 594__ $$a10.9$$b2022
000117646 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000117646 700__ $$aPostigo, Alejandro
000117646 700__ $$aLancelot, Alexandre
000117646 700__ $$0(orcid)0000-0001-9866-6633$$aSerrano, Jose Luis$$uUniversidad de Zaragoza
000117646 700__ $$0(orcid)0000-0001-7091-077X$$aSierra, Teresa
000117646 700__ $$0(orcid)0000-0003-3109-4284$$aHernandez-Ainsa, Silvia$$uUniversidad de Zaragoza
000117646 7102_ $$12013$$2765$$aUniversidad de Zaragoza$$bDpto. Química Orgánica$$cÁrea Química Orgánica
000117646 773__ $$g10 (2022), 2706-2719$$pBiomater. sci.$$tBiomaterials Science$$x2047-4830
000117646 8564_ $$s5050385$$uhttps://zaguan.unizar.es/record/117646/files/texto_completo.pdf$$yVersión publicada
000117646 8564_ $$s2880961$$uhttps://zaguan.unizar.es/record/117646/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000117646 909CO $$ooai:zaguan.unizar.es:117646$$particulos$$pdriver
000117646 951__ $$a2024-03-18-15:33:03
000117646 980__ $$aARTICLE