000117964 001__ 117964
000117964 005__ 20230519145608.0
000117964 0247_ $$2doi$$a10.3390/biomedicines9060682
000117964 0248_ $$2sideral$$a127250
000117964 037__ $$aART-2021-127250
000117964 041__ $$aeng
000117964 100__ $$aRodrigo M.J.$$uUniversidad de Zaragoza
000117964 245__ $$aChronic glaucoma using biodegradable microspheres to induce intraocular pressure elevation. Six-month follow-up
000117964 260__ $$c2021
000117964 5060_ $$aAccess copy available to the general public$$fUnrestricted
000117964 5203_ $$aBackground: To compare two prolonged animal models of glaucoma over 24 weeks of follow-up. A novel pre-trabecular model of chronic glaucoma was achieved by injection of biodegradable poly lactic-co-glycolic acid (PLGA) microspheres (10–20 µm) (Ms20/10) into the ocular anterior chamber to progressively increase ocular hypertension (OHT). Methods: Rat right eyes were injected to induce OHT: 50% received a suspension of Ms20/10 in the anterior chamber at 0, 2, 4, 8, 12, 16 and 20 weeks, and the other 50% received a sclerosing episcleral vein injection biweekly (EPIm). Ophthalmological clinical signs, intraocular pressure (IOP), neuroretinal functionality measured by electroretinography (ERG), and structural analysis of the retina, retinal nerve fiber layer (RNFL) and ganglion cell layer (GCL) protocols using optical coherence tomography (OCT) and histological exams were performed. Results: Both models showed progressive neuroretinal degeneration (p < 0.05), and contralateral eye affectation. The Ms20/10 model showed a more progressive increase in IOP and better preservation of ocular surface. Although no statistical differences were found between models, the EPIm showed a tendency to produce thicker retinal and thinner GCL thicknesses, slower latency and smaller amplitude as measured using ERG, and more aggressive disturbances in retinal histology. In both models, while the GCL showed the greatest percentage loss of thickness, the RNFL showed the greatest and earliest rate of thickness loss. Conclusions: The intracameral model with biodegradable microspheres resulted more like the conditions observed in humans. It was obtained by a less-aggressive mechanism, which allows for adequate study of the pathology over longer periods. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
000117964 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000117964 594__ $$a3.0$$b2021
000117964 590__ $$a4.757$$b2021
000117964 592__ $$a0.874$$b2021
000117964 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b121 / 297 = 0.407$$c2021$$dQ2$$eT2
000117964 593__ $$aMedicine (miscellaneous)$$c2021$$dQ1
000117964 591__ $$aPHARMACOLOGY & PHARMACY$$b87 / 279 = 0.312$$c2021$$dQ2$$eT1
000117964 593__ $$aBiochemistry, Genetics and Molecular Biology (miscellaneous)$$c2021$$dQ1
000117964 591__ $$aMEDICINE, RESEARCH & EXPERIMENTAL$$b62 / 140 = 0.443$$c2021$$dQ2$$eT2
000117964 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000117964 700__ $$aGarcia-Herranz D.
000117964 700__ $$aSubias M.
000117964 700__ $$aMartinez-Rincón T.$$uUniversidad de Zaragoza
000117964 700__ $$0(orcid)0000-0001-6072-1545$$aMendez-Martínez S.$$uUniversidad de Zaragoza
000117964 700__ $$aBravo-Osuna I.
000117964 700__ $$aCarretero A.
000117964 700__ $$aRuberte J.
000117964 700__ $$aGarcia-Feijoo J.
000117964 700__ $$0(orcid)0000-0003-2389-8282$$aPablo L.E.$$uUniversidad de Zaragoza
000117964 700__ $$aHerrero-Vanrell R.
000117964 700__ $$0(orcid)0000-0001-6258-2489$$aGarcia-Martin E.$$uUniversidad de Zaragoza
000117964 7102_ $$11013$$2645$$aUniversidad de Zaragoza$$bDpto. Cirugía$$cÁrea Obstetricia y Ginecología
000117964 7102_ $$11013$$2646$$aUniversidad de Zaragoza$$bDpto. Cirugía$$cÁrea Oftalmología
000117964 773__ $$g9, 6 (2021), 682 [26 pp]$$tBiomedicines$$x2227-9059
000117964 8564_ $$s7312446$$uhttps://zaguan.unizar.es/record/117964/files/texto_completo.pdf$$yVersión publicada
000117964 8564_ $$s2794334$$uhttps://zaguan.unizar.es/record/117964/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000117964 909CO $$ooai:zaguan.unizar.es:117964$$particulos$$pdriver
000117964 951__ $$a2023-05-18-16:06:00
000117964 980__ $$aARTICLE