000117986 001__ 117986
000117986 005__ 20240319081014.0
000117986 0247_ $$2doi$$a10.1038/s41598-022-10589-y
000117986 0248_ $$2sideral$$a128581
000117986 037__ $$aART-2022-128581
000117986 041__ $$aeng
000117986 100__ $$0(orcid)0000-0003-3580-1273$$aBailo, Rebeca$$uUniversidad de Zaragoza
000117986 245__ $$aThe mycobacterial desaturase DesA2 is associated with mycolic acid biosynthesis
000117986 260__ $$c2022
000117986 5060_ $$aAccess copy available to the general public$$fUnrestricted
000117986 5203_ $$aMycolic acids are critical for the survival and virulence of Mycobacterium tuberculosis, the causative agent of tuberculosis. Double bond formation in the merochain of mycolic acids remains poorly understood, though we have previously shown desA1, encoding an aerobic desaturase, is involved in mycolic acid desaturation. Here we show that a second desaturase encoded by desA2 is also involved in mycolate biosynthesis. DesA2 is essential for growth of the fast-growing Mycobacterium smegmatis in laboratory media. Conditional depletion of DesA2 led to a decrease in mycolic acid biosynthesis and loss of mycobacterial viability. Additionally, DesA2-depleted cells also accumulated fatty acids of chain lengths C19-C24. The complete loss of mycolate biosynthesis following DesA2 depletion, and the absence of any monoenoic derivatives (found to accumulate on depletion of DesA1) suggests an early role for DesA2 in the mycolic acid biosynthesis machinery, highlighting its potential as a drug target.
000117986 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000117986 590__ $$a4.6$$b2022
000117986 592__ $$a0.973$$b2022
000117986 591__ $$aMULTIDISCIPLINARY SCIENCES$$b22 / 73 = 0.301$$c2022$$dQ2$$eT1
000117986 593__ $$aMultidisciplinary$$c2022$$dQ1
000117986 594__ $$a7.5$$b2022
000117986 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000117986 700__ $$aRadhakrishnan, Anjana
000117986 700__ $$aSingh, Albel
000117986 700__ $$aNakaya, Makoto
000117986 700__ $$aFujiwara, Nagatoshi
000117986 700__ $$aBhatt, Apoorva
000117986 7102_ $$11011$$2630$$aUniversidad de Zaragoza$$bDpto. Microb.Ped.Radio.Sal.Pú.$$cÁrea Microbiología
000117986 773__ $$g12, 1 (2022), 6943 [10 pp.]$$pSci. rep. (Nat. Publ. Group)$$tScientific reports (Nature Publishing Group)$$x2045-2322
000117986 8564_ $$s3832883$$uhttps://zaguan.unizar.es/record/117986/files/texto_completo.pdf$$yVersión publicada
000117986 8564_ $$s2767725$$uhttps://zaguan.unizar.es/record/117986/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000117986 909CO $$ooai:zaguan.unizar.es:117986$$particulos$$pdriver
000117986 951__ $$a2024-03-18-15:26:27
000117986 980__ $$aARTICLE