000118013 001__ 118013
000118013 005__ 20240319080945.0
000118013 0247_ $$2doi$$a10.1038/s41467-022-32350-9
000118013 0248_ $$2sideral$$a129511
000118013 037__ $$aART-2022-129511
000118013 041__ $$aeng
000118013 100__ $$aGracia, Pablo
000118013 245__ $$aMolecular mechanism for the synchronized electrostatic coacervation and co-aggregation of a-synuclein and tau
000118013 260__ $$c2022
000118013 5060_ $$aAccess copy available to the general public$$fUnrestricted
000118013 5203_ $$aAmyloid aggregation of α-synuclein (αS) is the hallmark of Parkinson’s disease and other synucleinopathies. Recently, Tau protein, generally associated with Alzheimer’s disease, has been linked to αS pathology and observed to co- localize in αS-rich disease inclusions, although the molecular mechanisms for the co-aggregation of both proteins remain elusive. We report here that αS phase-separates into liquid condensates by electrostatic complex coacerva- tion with positively charged polypeptides such as Tau. Condensates undergo either fast gelation or coalescence followed by slow amyloid aggregation depending on the affinity of αS for the poly-cation and the rate of valence exhaustion of the condensate network. By combining a set of advanced bio- physical techniques, we have been able to characterize αS/Tau liquid-liquid phase separation and identified key factors that lead to the formation of hetero-aggregates containing both proteins in the interior of the liquid protein condensates.
000118013 536__ $$9info:eu-repo/grantAgreement/EC/H2020/813209/EU/Paramagnetic Species in Catalysis Research. A Unified Approach Towards Heterogeneous, Homogeneous and Enzyme Catalysis/PARACAT$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 813209-PARACAT$$9info:eu-repo/grantAgreement/ES/MICINN-AEI/PGC2018-096335-B100$$9info:eu-repo/grantAgreement/ES/MICINN-AEI/PID2019-109276RA-I00$$9info:eu-repo/grantAgreement/ES/MICINN-AEI/PID2019-109306RB-I00$$9info:eu-repo/grantAgreement/ES/MICINN/RYC2018-026042-I$$9info:eu-repo/grantAgreement/ES/UZ/CUD2019-BIO-01$$9info:eu-repo/grantAgreement/ES/UZ/CUD2020-BIO-01
000118013 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000118013 590__ $$a16.6$$b2022
000118013 592__ $$a5.116$$b2022
000118013 591__ $$aMULTIDISCIPLINARY SCIENCES$$b6 / 73 = 0.082$$c2022$$dQ1$$eT1
000118013 593__ $$aBiochemistry, Genetics and Molecular Biology (miscellaneous)$$c2022$$dQ1
000118013 593__ $$aPhysics and Astronomy (miscellaneous)$$c2022$$dQ1
000118013 593__ $$aChemistry (miscellaneous)$$c2022$$dQ1
000118013 594__ $$a24.9$$b2022
000118013 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000118013 700__ $$0(orcid)0000-0002-0005-6712$$aPolanco, David$$uUniversidad de Zaragoza
000118013 700__ $$aTarancón-Díez, Jorge
000118013 700__ $$aSerra, Ilenia
000118013 700__ $$0(orcid)0000-0001-8310-8970$$aBracci, Maruan$$uUniversidad de Zaragoza
000118013 700__ $$aOroz, Javier
000118013 700__ $$aLaurents, Douglas V.
000118013 700__ $$0(orcid)0000-0002-1827-1250$$aGarcía-Rubio, Inés$$uUniversidad de Zaragoza
000118013 700__ $$0(orcid)0000-0002-9138-6687$$aCremades, Nunilo$$uUniversidad de Zaragoza
000118013 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000118013 7102_ $$12003$$2395$$aUniversidad de Zaragoza$$bDpto. Física Materia Condensa.$$cÁrea Física Materia Condensada
000118013 773__ $$g13 (2022), 4586 [16 pp.]$$tNature communications$$x2041-1723
000118013 8564_ $$s4962757$$uhttps://zaguan.unizar.es/record/118013/files/texto_completo.pdf$$yVersión publicada
000118013 8564_ $$s2671764$$uhttps://zaguan.unizar.es/record/118013/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000118013 909CO $$ooai:zaguan.unizar.es:118013$$particulos$$pdriver
000118013 951__ $$a2024-03-18-12:30:21
000118013 980__ $$aARTICLE