118018 20240319080953.0 doi 10.1186/s40850-022-00124-x sideral 128702 ART-2022-128702 eng Casades-Martí, L. Confirmed presence of aedes (rusticoidus) refiki Medschid, 1928 in a continental dry Mediterranean peri-urban environment in south-central Spain 2022 Access copy available to the general public Unrestricted Background: The ‘snow-melt mosquito’ aedes (rusticoidus) refiki is a rare species with a wide distribution in Europe that is usually defined as an aggressive mosquito for mammals, including humans. During a mosquito survey in a peri-urban area in south-central mainland Spain, adult Ae. refiki females were captured and identified by morphological traits. The presence of this species of mosquito has never been molecularly confirmed under continental dry Mediterranean climatic influence with scarce number of days with snow on soil. The aim of this study was to confirm by amplification and sequencing of mitochondrial cytochrome c oxidase subunit I (COI) and internal transcribed spacer 2 (ITS2) region. Results: We also successfully amplified and typed the species molecularly by COI and ITS2 regions. The peri-urban area where Ae. refiki was found contrasts with the reported cold, humid and snowy environments required by the species to breed. Conclusions: This finding suggests that the species is already adapted to continental dry Mediterranean environments, questioning whether it is a truly stenotopic species of cold snowy environments. info:eu-repo/grantAgreement/ES/MCIU/CD18-00091 info:eu-repo/grantAgreement/ES/MICINN/RTA2015-00002-C02-02 info:eu-repo/grantAgreement/ES/MINECO/CGL2017-89866-R info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 1.6 2022 ZOOLOGY 63 / 176 = 0.358 2022 Q2 T2 0.453 2022 Animal Science and Zoology 2022 Q2 1.7 2022 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Frías, M. Delacour, S. Universidad de Zaragoza (orcid)0000-0001-7046-2997 Ruiz-Fons, F. 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 7 (2022), 21 [7 pp.] BMC Zoology 2056-3132 1661404 http://zaguan.unizar.es/record/118018/files/texto_completo.pdf Versión publicada 2361847 http://zaguan.unizar.es/record/118018/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:118018 articulos driver 2024-03-18-13:14:44 ARTICLE