118052 20220831113014.0 doi 10.1016/j.microc.2019.104040 sideral 112952 ART-2019-112952 eng Abad-Alvaro, I. (orcid)0000-0002-7931-3901 Silver nanoparticles-clays nanocomposites as feed additives: Characterization of silver species released during in vitro digestions. Effects on silver retention in pigs 2019 Two different clay nanocomposites, as sepiolite-Ag and kaolinite-Ag, are studied as carriers for silver nano particles (AgNPs) oral administration as antimicrobial agent in additives for animal feed. A three-step digestibility assay, corresponding to stomach, small and large intestine simulations, has been followed. Ultrafiltration and asymmetrical flow field-flow fractionation (AF4) coupled to UV-Vis absorption and ICPMS detectors have been used for size characterisation of the silver species released during the in vitro digestibility assays. Less than 1% of the total silver is released in the stomach simulation step, probably due to the formation of silver chloride on the nanocomposite surface. In the case of the intestine simulation, silver released increases and tends to form complexes with the enzymes present in the media. A larger amount of silver was released from kaolinite-Ag compared to sepiolite-Ag (17 vs. 7%), probably due to a higher retention rate of silver shown by sepiolite, justified by its sorption capacity and fibrous structure. No evidences were found about a significant release of silver nanoparticles along the in vitro assay from any of the two nanocomposites studied. These results have been compared to the silver levels found in liver and faeces from weaned pigs fed with these supplements for 35 days and followed by basal diet free of silver for 4 weeks in an in vivo assay. Piglets fed with kaolinite-Ag retained more silver in liver than those receiving sepiolite-Ag, and the opposite was observed in faeces, although differences were not statistically significant. Silver levels found in muscles were below the limit of detection (0.009 mu g Ag g(-1)) in all cases. Access copy available to the general public Unrestricted info:eu-repo/grantAgreement/ES/DGA/E29-17R info:eu-repo/grantAgreement/ES/MINECO-FEDER/CTQ2015-68094-C2-1-R info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 3.594 2019 CHEMISTRY, ANALYTICAL 19 / 86 = 0.221 2019 Q1 T1 0.657 2019 Spectroscopy 2019 Q2 Analytical Chemistry 2019 Q2 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Trujillo, C. Universidad de Zaragoza (orcid)0000-0002-0341-9662 Bolea, E. Universidad de Zaragoza (orcid)0000-0001-5382-0561 Laborda, F. Universidad de Zaragoza (orcid)0000-0002-4169-0357 Fondevila, M. Universidad de Zaragoza (orcid)0000-0002-0712-1185 Latorre, M.A. Universidad de Zaragoza (orcid)0000-0002-3005-2675 Castillo, J.R. Universidad de Zaragoza (orcid)0000-0002-4070-8607 2009 750 Universidad de Zaragoza Dpto. Química Analítica Área Química Analítica 2008 700 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Producción Animal 149 (2019), 104040 [9 pp.] Microchem. j. MICROCHEMICAL JOURNAL 0026-265X 2810000 http://zaguan.unizar.es/record/118052/files/texto_completo.pdf Postprint 1798749 http://zaguan.unizar.es/record/118052/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:118052 articulos driver 2022-08-31-09:13:36 ARTICLE