000118181 001__ 118181
000118181 005__ 20240319081011.0
000118181 0247_ $$2doi$$a10.3389/fmed.2022.875147
000118181 0248_ $$2sideral$$a129375
000118181 037__ $$aART-2022-129375
000118181 041__ $$aeng
000118181 100__ $$0(orcid)0000-0001-8056-5236$$aSolanas, Estela$$uUniversidad de Zaragoza
000118181 245__ $$aHow Donor and Surgical Factors Affect the Viability and Functionality of Human Hepatocytes Isolated From Liver Resections
000118181 260__ $$c2022
000118181 5060_ $$aAccess copy available to the general public$$fUnrestricted
000118181 5203_ $$aLiver resections are a significant source of primary human hepatocytes used mainly in artificial liver devices and pharmacological and biomedical studies. However, it is not well known how patient-donor and surgery-dependent factors influence isolated hepatocytes’ yield, viability, and function. Hence, we aimed to analyze the impact of all these elements on the outcome of human hepatocyte isolation.
Patients and methods: Hepatocytes were isolated from liver tissue from patients undergoing partial hepatectomy using a two-step collagenase method. Hepatocyte viability, cell yield, adhesion, and functionality were measured. In addition, clinical and analytical patient variables were collected and the use or absence of vascular clamping and its type (continuous or intermittent) plus the ischemia times during surgery.
Results: Malignant disease, previous chemotherapy, and male gender were associated with lower hepatocyte viability and isolation cell yields. The previous increase in transaminases was also associated with lower yields on isolation and lower albumin production. Furthermore, ischemia secondary to vascular clamping during surgery was inversely correlated with the isolated hepatocyte viability. An ischemia time higher than 15 min was related to adverse effects on viability.
Conclusion: Several factors correlated with the patient and the surgery directly influence the success of human hepatocyte isolation from patients undergoing liver resection.
000118181 536__ $$9info:eu-repo/grantAgreement/ES/ISCIII/FIS/PI18-00529$$9info:eu-repo/grantAgreement/ES/ISCIII/PI21-00667
000118181 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000118181 590__ $$a3.9$$b2022
000118181 592__ $$a0.926$$b2022
000118181 591__ $$aMEDICINE, GENERAL & INTERNAL$$b58 / 169 = 0.343$$c2022$$dQ2$$eT2
000118181 593__ $$aMedicine (miscellaneous)$$c2022$$dQ1
000118181 594__ $$a3.6$$b2022
000118181 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000118181 700__ $$aSanchez-Fuentes, Nieves
000118181 700__ $$0(orcid)0000-0003-4712-9891$$aSerrablo, Alejandro$$uUniversidad de Zaragoza
000118181 700__ $$aLue, Alberto$$uUniversidad de Zaragoza
000118181 700__ $$0(orcid)0000-0003-4672-8083$$aLorente, Sara$$uUniversidad de Zaragoza
000118181 700__ $$aCortés, Luis$$uUniversidad de Zaragoza
000118181 700__ $$0(orcid)0000-0001-5932-2889$$aLanas, Angel$$uUniversidad de Zaragoza
000118181 700__ $$0(orcid)0000-0002-8969-7726$$aBaptista, Pedro M.
000118181 700__ $$0(orcid)0000-0002-7119-2244$$aSerrano, M. Trinidad$$uUniversidad de Zaragoza
000118181 7102_ $$11003$$2027$$aUniversidad de Zaragoza$$bDpto. Anatom.Histolog.Humanas$$cArea Anatom.Embriol.Humana
000118181 7102_ $$11007$$2610$$aUniversidad de Zaragoza$$bDpto. Medicina, Psiqu. y Derm.$$cArea Medicina
000118181 7102_ $$11013$$2090$$aUniversidad de Zaragoza$$bDpto. Cirugía$$cÁrea Cirugía
000118181 773__ $$g9 (2022), 875147 [9 pp.]$$pFront. med.$$tFrontiers in Medicine$$x2296-858X
000118181 8564_ $$s292440$$uhttps://zaguan.unizar.es/record/118181/files/texto_completo.pdf$$yVersión publicada
000118181 8564_ $$s2067119$$uhttps://zaguan.unizar.es/record/118181/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000118181 909CO $$ooai:zaguan.unizar.es:118181$$particulos$$pdriver
000118181 951__ $$a2024-03-18-15:11:48
000118181 980__ $$aARTICLE