118181 20240319081011.0 doi 10.3389/fmed.2022.875147 sideral 129375 ART-2022-129375 eng Solanas, Estela Universidad de Zaragoza (orcid)0000-0001-8056-5236 How Donor and Surgical Factors Affect the Viability and Functionality of Human Hepatocytes Isolated From Liver Resections 2022 Access copy available to the general public Unrestricted Liver resections are a significant source of primary human hepatocytes used mainly in artificial liver devices and pharmacological and biomedical studies. However, it is not well known how patient-donor and surgery-dependent factors influence isolated hepatocytes’ yield, viability, and function. Hence, we aimed to analyze the impact of all these elements on the outcome of human hepatocyte isolation. Patients and methods: Hepatocytes were isolated from liver tissue from patients undergoing partial hepatectomy using a two-step collagenase method. Hepatocyte viability, cell yield, adhesion, and functionality were measured. In addition, clinical and analytical patient variables were collected and the use or absence of vascular clamping and its type (continuous or intermittent) plus the ischemia times during surgery. Results: Malignant disease, previous chemotherapy, and male gender were associated with lower hepatocyte viability and isolation cell yields. The previous increase in transaminases was also associated with lower yields on isolation and lower albumin production. Furthermore, ischemia secondary to vascular clamping during surgery was inversely correlated with the isolated hepatocyte viability. An ischemia time higher than 15 min was related to adverse effects on viability. Conclusion: Several factors correlated with the patient and the surgery directly influence the success of human hepatocyte isolation from patients undergoing liver resection. info:eu-repo/grantAgreement/ES/ISCIII/FIS/PI18-00529 info:eu-repo/grantAgreement/ES/ISCIII/PI21-00667 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 3.9 2022 MEDICINE, GENERAL & INTERNAL 58 / 169 = 0.343 2022 Q2 T2 0.926 2022 Medicine (miscellaneous) 2022 Q1 3.6 2022 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Sanchez-Fuentes, Nieves Serrablo, Alejandro Universidad de Zaragoza (orcid)0000-0003-4712-9891 Lue, Alberto Universidad de Zaragoza Lorente, Sara Universidad de Zaragoza (orcid)0000-0003-4672-8083 Cortés, Luis Universidad de Zaragoza Lanas, Angel Universidad de Zaragoza (orcid)0000-0001-5932-2889 Baptista, Pedro M. (orcid)0000-0002-8969-7726 Serrano, M. Trinidad Universidad de Zaragoza (orcid)0000-0002-7119-2244 1003 027 Universidad de Zaragoza Dpto. Anatom.Histolog.Humanas Area Anatom.Embriol.Humana 1007 610 Universidad de Zaragoza Dpto. Medicina, Psiqu. y Derm. Area Medicina 1013 090 Universidad de Zaragoza Dpto. Cirugía Área Cirugía 9 (2022), 875147 [9 pp.] Front. med. Frontiers in Medicine 2296-858X 292440 http://zaguan.unizar.es/record/118181/files/texto_completo.pdf Versión publicada 2067119 http://zaguan.unizar.es/record/118181/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:118181 articulos driver 2024-03-18-15:11:48 ARTICLE