000118195 001__ 118195
000118195 005__ 20240319081020.0
000118195 0247_ $$2doi$$a10.1186/s13071-022-05329-0
000118195 0248_ $$2sideral$$a129584
000118195 037__ $$aART-2022-129584
000118195 041__ $$aeng
000118195 100__ $$aHernández-Triana, L. M.
000118195 245__ $$aSusceptibility of Aedes albopictus and Culex quinquefasciatus to Japanese encephalitis virus; 35710580
000118195 260__ $$c2022
000118195 5060_ $$aAccess copy available to the general public$$fUnrestricted
000118195 5203_ $$aBackground: Japanese encephalitis virus (JEV) is the principal cause of mosquito-borne encephalitis in human populations within Asia. If introduced into new geographic areas, it could have further implications for public and animal health. However, potential mosquito vectors for virus transmission have not been fully investigated. The Asian tiger mosquito, Aedes albopictus, has emerged in Europe and is now expanding its geographical range into more northerly latitudes. Culex quinquefasciatus, although absent from Europe, has been detected in Turkey, a country with territory in Europe, and could act as a vector for JEV in other regions. To assess the risk of these invasive species acting as vectors for JEV and therefore potentially contributing to its geographical expansion, we have investigated the vector competence of Ae. albopictus and Cx. quinquefasciatus. Methods: Two colonised lines of Ae. albopictus (Italy and Spain) and a line of Cx. quinquefasciatus (Tanzania) were compared for susceptibility to infection by oral feeding with JEV strain SA-14, genotype III at 106 PFU/ml and maintained at 25 °C. Specimens were processed at 7 and 14 days post-inoculation (dpi). Rates of infection, dissemination and transmission were assessed through detection of viral RNA by real-time polymerase chain reaction (RT-PCR) in mosquito body, legs and saliva, respectively, at each time point. Where possible, infection and dissemination were confirmed by immunohistochemical (IHC) detection of the JEV envelope protein. Results: Aedes albopictus from Italy showed no susceptibility to infection with JEV strain SA-14. Conversely, Ae. albopictus colonised in Spain was susceptible and 100% of infected mosquitoes that were subjected to saliva screening expressed viral RNA at 14 dpi. Culex quinquefasciatus was highly susceptible to infection as early as 7 dpi and 50% of infected mosquitoes that were subjected to saliva screening expressed viral RNA at 14 dpi. Infection and dissemination were confirmed in Cx. quinquefasciatus by IHC detection of JEV envelope protein in both the mid-gut and salivary glands. Conclusions: Aedes albopictus from two different locations in Europe range from being susceptible to JEV and capable of transmission through to being resistant. Culex quinquefasciatus also appears highly susceptible; therefore, both species could potentially act as vectors for JEV and facilitate the emergence of JEV into new regions. Graphical Abstract: Figure not available: see fulltext.] © 2022, The Author(s).
000118195 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000118195 590__ $$a3.2$$b2022
000118195 592__ $$a0.961$$b2022
000118195 591__ $$aTROPICAL MEDICINE$$b6 / 24 = 0.25$$c2022$$dQ1$$eT1
000118195 593__ $$aParasitology$$c2022$$dQ1
000118195 591__ $$aPARASITOLOGY$$b9 / 37 = 0.243$$c2022$$dQ1$$eT1
000118195 593__ $$aInfectious Diseases$$c2022$$dQ2
000118195 594__ $$a6.6$$b2022
000118195 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000118195 700__ $$aFolly, A. J.
000118195 700__ $$aSewgobind, S.
000118195 700__ $$aLean, F. Z. X.
000118195 700__ $$aAckroyd, S.
000118195 700__ $$aNuñez, A.
000118195 700__ $$0(orcid)0000-0001-7046-2997$$aDelacour Estrella, S.$$uUniversidad de Zaragoza
000118195 700__ $$aDrago, A.
000118195 700__ $$aVisentin, P.
000118195 700__ $$aMansfield, K. L.
000118195 700__ $$aJohnson, N.
000118195 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000118195 773__ $$g15, 1 (2022), 210 [8 pp]$$pParasites & Vectors$$tParasites and Vectors$$x1756-3305
000118195 8564_ $$s1068375$$uhttps://zaguan.unizar.es/record/118195/files/texto_completo.pdf$$yVersión publicada
000118195 8564_ $$s2497854$$uhttps://zaguan.unizar.es/record/118195/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000118195 909CO $$ooai:zaguan.unizar.es:118195$$particulos$$pdriver
000118195 951__ $$a2024-03-18-16:04:33
000118195 980__ $$aARTICLE