000118206 001__ 118206
000118206 005__ 20240319081021.0
000118206 0247_ $$2doi$$a10.3390/cells11111791
000118206 0248_ $$2sideral$$a129602
000118206 037__ $$aART-2022-129602
000118206 041__ $$aeng
000118206 100__ $$0(orcid)0000-0001-5573-6144$$aLayunta, E.$$uUniversidad de Zaragoza
000118206 245__ $$aTLR2 and TLR4 Modulate Mouse Ileal Motility by the Interaction with Muscarinic and Nicotinic Receptors; 35681486
000118206 260__ $$c2022
000118206 5060_ $$aAccess copy available to the general public$$fUnrestricted
000118206 5203_ $$aIrritable bowel syndrome (IBS) is a chronic functional bowel disorder characterized by intestinal dysmotility. Changes in intestinal microbiota (dysbiosis) can lead to alterations in neuro-muscular functions in the gut. Toll-like receptors (TLRs) 2 and 4 recognize intestinal bacteria and are involved in the motor response induced by gastrointestinal (GI) neurotransmitters. Acetylcholine (ACh) is a well-known neurotransmitter involved in the regulation of GI motility. This study aimed to evaluate the role of TLR2 and TLR4 in the intestinal motor-response induced by ACh in the mouse ileum, as well as the expression and function of the muscarinic and nicotinic ACh receptors. Muscle contractility studies showed that the contractions induced by ACh were significantly lower in TLR2-/- and TLR4-/- with respect to WT mice. In WT mice, the contractions induced by ACh were reduced in the presence of AF-DX AF-DX 116 (a muscarinic ACh receptor (mAChR) M2 antagonist), 4-DAMP (a mAChR M3 antagonist), mecamylamine (a nicotinic AChR receptor (nAChR) a3ß4 antagonist) and a-bungarotoxin (a nAChR a7 antagonist). In TLR2-/- mice, the contractions induced by ACh were increased by AF-DX 116 and mecamylamine. In TLR4-/- mice, the contractions induced by ACh were reduced by a-bungarotoxin and 4-DAMP. The mRNA and protein expressions of M3 and a3 receptors were diminished in the ileum from TLR2-/- and TLR4-/- with respect to WT mice. However, the levels of mRNA and protein of ß4 were diminished only in TLR4-/- but not in TLR2-/- mice. In conclusion, our results show that TLR2 and TLR4 modulates the motor responses to ACh in the mouse ileum. TLR2 acts on muscarinic M2 and M3 and nicotinic a3ß4 ACh receptors, while TLR4 acts on muscarinic M3 and nicotinic a3ß4 and a7 ACh receptors.
000118206 536__ $$9info:eu-repo/grantAgreement/ES/DGA/B61
000118206 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000118206 590__ $$a6.0$$b2022
000118206 592__ $$a1.537$$b2022
000118206 591__ $$aCELL BIOLOGY$$b60 / 191 = 0.314$$c2022$$dQ2$$eT1
000118206 593__ $$aBiochemistry, Genetics and Molecular Biology (miscellaneous)$$c2022$$dQ1
000118206 594__ $$a9.0$$b2022
000118206 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000118206 700__ $$aForcén, R.
000118206 700__ $$0(orcid)0000-0002-5306-9365$$aGrasa López, L.$$uUniversidad de Zaragoza
000118206 7102_ $$11012$$2410$$aUniversidad de Zaragoza$$bDpto. Farmac.Fisiol.y Med.L.F.$$cÁrea Fisiología
000118206 773__ $$g11, 11 (2022), 1791 [13 pp]$$pCells$$tCells$$x2073-4409
000118206 8564_ $$s2647491$$uhttps://zaguan.unizar.es/record/118206/files/texto_completo.pdf$$yVersión publicada
000118206 8564_ $$s2750342$$uhttps://zaguan.unizar.es/record/118206/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000118206 909CO $$ooai:zaguan.unizar.es:118206$$particulos$$pdriver
000118206 951__ $$a2024-03-18-16:12:52
000118206 980__ $$aARTICLE