000118269 001__ 118269
000118269 005__ 20240319081010.0
000118269 0247_ $$2doi$$a10.3390/ijms23137182
000118269 0248_ $$2sideral$$a129395
000118269 037__ $$aART-2022-129395
000118269 041__ $$aeng
000118269 100__ $$0(orcid)0000-0002-0388-9429$$aBetancor, M.$$uUniversidad de Zaragoza
000118269 245__ $$aNeurogranin and Neurofilament Light Chain as Preclinical Biomarkers in Scrapie
000118269 260__ $$c2022
000118269 5060_ $$aAccess copy available to the general public$$fUnrestricted
000118269 5203_ $$aPrion diseases are diagnosed in the symptomatic stage, when the neuronal damage is spread throughout the central nervous system (CNS). The assessment of biological features that allow the detection of asymptomatic cases is needed, and, in this context, scrapie, where pre-symptomatic infected animals can be detected through rectal biopsy, becomes a good study model. Neurogranin (Ng) and neurofilament light chain (NfL) are proteins that reflect synaptic and axonal damage and have been studied as cerebrospinal fluid (CSF) biomarkers in different neurodegenerative disorders. In this study, we evaluated Ng and NfL both at the protein and transcript levels in the CNS of preclinical and clinical scrapie-affected sheep compared with healthy controls and assessed their levels in ovine CSF. The correlation between these proteins and the main neuropathological events in prion diseases, PrPSc deposition and spongiosis, was also assessed. The results show a decrease in Ng and NfL at the protein and gene expression levels as the disease progresses, and significant changes between the control and preclinical animals. On the contrary, the CSF levels of NfL increased throughout the progression of the disease. Negative correlations between neuropathological markers of prion disease and the concentration of the studied proteins were also found. Although further research is needed, these results suggest that Ng and NfL could act as biomarkers for neurodegeneration onset and intensity in preclinical cases of scrapie.
000118269 536__ $$9info:eu-repo/grantAgreement/ES/DGA-FSE/IIU-2023-2017$$9info:eu-repo/grantAgreement/EC/H2020/860197/EU/Multi-omics Interdisciplinary Research Integration to Address DEmentia diagnosis/MIRIADE$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 860197-MIRIADE$$9info:eu-repo/grantAgreement/EUR/INTERREG-POCTEFA/EFA148-16$$9info:eu-repo/grantAgreement/ES/MICINN/RTI2018-098711-B-I00
000118269 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000118269 590__ $$a5.6$$b2022
000118269 592__ $$a1.154$$b2022
000118269 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b66 / 285 = 0.232$$c2022$$dQ1$$eT1
000118269 593__ $$aMedicine (miscellaneous)$$c2022$$dQ1
000118269 591__ $$aCHEMISTRY, MULTIDISCIPLINARY$$b52 / 178 = 0.292$$c2022$$dQ2$$eT1
000118269 593__ $$aPhysical and Theoretical Chemistry$$c2022$$dQ1
000118269 593__ $$aComputer Science Applications$$c2022$$dQ1
000118269 593__ $$aInorganic Chemistry$$c2022$$dQ1
000118269 593__ $$aSpectroscopy$$c2022$$dQ1
000118269 593__ $$aOrganic Chemistry$$c2022$$dQ1
000118269 593__ $$aMolecular Biology$$c2022$$dQ2
000118269 593__ $$aCatalysis$$c2022$$dQ2
000118269 594__ $$a7.8$$b2022
000118269 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000118269 700__ $$0(orcid)0000-0002-2079-0711$$aPérez-Lázaro, S.$$uUniversidad de Zaragoza
000118269 700__ $$0(orcid)0000-0001-9075-2764$$aOtero, A.$$uUniversidad de Zaragoza
000118269 700__ $$0(orcid)0000-0002-1590-3347$$aMarín, B.$$uUniversidad de Zaragoza
000118269 700__ $$0(orcid)0000-0001-6016-4726$$aMartín-Burriel , I.$$uUniversidad de Zaragoza
000118269 700__ $$aBlennow, K.
000118269 700__ $$0(orcid)0000-0002-7173-7216$$aBadiola, J.J.$$uUniversidad de Zaragoza
000118269 700__ $$aZetterberg, H.
000118269 700__ $$0(orcid)0000-0002-2746-3932$$aBolea, R.$$uUniversidad de Zaragoza
000118269 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000118269 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000118269 773__ $$g23, 13 (2022), 7182 [16 pp.]$$pInt. j. mol. sci.$$tInternational Journal of Molecular Sciences$$x1661-6596
000118269 8564_ $$s849426$$uhttps://zaguan.unizar.es/record/118269/files/texto_completo.pdf$$yVersión publicada
000118269 8564_ $$s2679173$$uhttps://zaguan.unizar.es/record/118269/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000118269 909CO $$ooai:zaguan.unizar.es:118269$$particulos$$pdriver
000118269 951__ $$a2024-03-18-15:04:54
000118269 980__ $$aARTICLE