118269 20240319081010.0 doi 10.3390/ijms23137182 sideral 129395 ART-2022-129395 eng Betancor, M. Universidad de Zaragoza (orcid)0000-0002-0388-9429 Neurogranin and Neurofilament Light Chain as Preclinical Biomarkers in Scrapie 2022 Access copy available to the general public Unrestricted Prion diseases are diagnosed in the symptomatic stage, when the neuronal damage is spread throughout the central nervous system (CNS). The assessment of biological features that allow the detection of asymptomatic cases is needed, and, in this context, scrapie, where pre-symptomatic infected animals can be detected through rectal biopsy, becomes a good study model. Neurogranin (Ng) and neurofilament light chain (NfL) are proteins that reflect synaptic and axonal damage and have been studied as cerebrospinal fluid (CSF) biomarkers in different neurodegenerative disorders. In this study, we evaluated Ng and NfL both at the protein and transcript levels in the CNS of preclinical and clinical scrapie-affected sheep compared with healthy controls and assessed their levels in ovine CSF. The correlation between these proteins and the main neuropathological events in prion diseases, PrPSc deposition and spongiosis, was also assessed. The results show a decrease in Ng and NfL at the protein and gene expression levels as the disease progresses, and significant changes between the control and preclinical animals. On the contrary, the CSF levels of NfL increased throughout the progression of the disease. Negative correlations between neuropathological markers of prion disease and the concentration of the studied proteins were also found. Although further research is needed, these results suggest that Ng and NfL could act as biomarkers for neurodegeneration onset and intensity in preclinical cases of scrapie. info:eu-repo/grantAgreement/ES/DGA-FSE/IIU-2023-2017 info:eu-repo/grantAgreement/EC/H2020/860197/EU/Multi-omics Interdisciplinary Research Integration to Address DEmentia diagnosis/MIRIADE This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 860197-MIRIADE info:eu-repo/grantAgreement/EUR/INTERREG-POCTEFA/EFA148-16 info:eu-repo/grantAgreement/ES/MICINN/RTI2018-098711-B-I00 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 5.6 2022 BIOCHEMISTRY & MOLECULAR BIOLOGY 66 / 285 = 0.232 2022 Q1 T1 CHEMISTRY, MULTIDISCIPLINARY 52 / 178 = 0.292 2022 Q2 T1 1.154 2022 Medicine (miscellaneous) 2022 Q1 Physical and Theoretical Chemistry 2022 Q1 Computer Science Applications 2022 Q1 Inorganic Chemistry 2022 Q1 Spectroscopy 2022 Q1 Organic Chemistry 2022 Q1 Molecular Biology 2022 Q2 Catalysis 2022 Q2 7.8 2022 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Pérez-Lázaro, S. Universidad de Zaragoza (orcid)0000-0002-2079-0711 Otero, A. Universidad de Zaragoza (orcid)0000-0001-9075-2764 Marín, B. Universidad de Zaragoza (orcid)0000-0002-1590-3347 Martín-Burriel , I. Universidad de Zaragoza (orcid)0000-0001-6016-4726 Blennow, K. Badiola, J.J. Universidad de Zaragoza (orcid)0000-0002-7173-7216 Zetterberg, H. Bolea, R. Universidad de Zaragoza (orcid)0000-0002-2746-3932 1001 420 Universidad de Zaragoza Dpto. Anatom.,Embri.Genét.Ani. Área Genética 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 23, 13 (2022), 7182 [16 pp.] Int. j. mol. sci. International Journal of Molecular Sciences 1661-6596 849426 http://zaguan.unizar.es/record/118269/files/texto_completo.pdf Versión publicada 2679173 http://zaguan.unizar.es/record/118269/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:118269 articulos driver 2024-03-18-15:04:54 ARTICLE