000118290 001__ 118290
000118290 005__ 20240319081022.0
000118290 0247_ $$2doi$$a10.3390/ijms23126786
000118290 0248_ $$2sideral$$a129628
000118290 037__ $$aART-2022-129628
000118290 041__ $$aeng
000118290 100__ $$aMocciaro, G.
000118290 245__ $$aLipidomic approaches to study HDL metabolism in patients with central obesity diagnosed with metabolic syndrome
000118290 260__ $$c2022
000118290 5060_ $$aAccess copy available to the general public$$fUnrestricted
000118290 5203_ $$aThe metabolic syndrome (MetS) is a cluster of cardiovascular risk factors characterised by central obesity, atherogenic dyslipidaemia, and changes in the circulating lipidome; the underlying mechanisms that lead to this lipid remodelling have only been partially elucidated. This study used an integrated “omics” approach (untargeted whole serum lipidomics, targeted proteomics, and lipoprotein lipidomics) to study lipoprotein remodelling and HDL composition in subjects with central obesity diagnosed with MetS (vs. controls). Compared with healthy subjects, MetS patients showed higher free fatty acids, diglycerides, phosphatidylcholines, and triglycerides, particularly those enriched in products of de novo lipogenesis. On the other hand, the “lysophosphatidylcholines to phosphatidylcholines” and “cholesteryl ester to free cholesterol” ratios were reduced, pointing to a lower activity of lecithin cholesterol acyltransferase (LCAT) in MetS; LCAT activity (directly measured and predicted by lipidomic ratios) was positively correlated with high-density lipoprotein cholesterol (HDL-C) and negatively correlated with body mass index (BMI) and insulin resistance. Moreover, many phosphatidylcholines and sphingomyelins were significantly lower in the HDL of MetS patients and strongly correlated with BMI and clinical metabolic parameters. These results suggest that MetS is associated with an impairment of phospholipid metabolism in HDL, partially led by LCAT, and associated with obesity and underlying insulin resistance. This study proposes a candidate strategy to use integrated “omics” approaches to gain mechanistic insights into lipoprotein remodelling, thus deepening the knowledge regarding the molecular basis of the association between MetS and atherosclerosis.
000118290 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000118290 590__ $$a5.6$$b2022
000118290 592__ $$a1.154$$b2022
000118290 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b66 / 285 = 0.232$$c2022$$dQ1$$eT1
000118290 593__ $$aMedicine (miscellaneous)$$c2022$$dQ1
000118290 591__ $$aCHEMISTRY, MULTIDISCIPLINARY$$b52 / 178 = 0.292$$c2022$$dQ2$$eT1
000118290 593__ $$aPhysical and Theoretical Chemistry$$c2022$$dQ1
000118290 593__ $$aComputer Science Applications$$c2022$$dQ1
000118290 593__ $$aInorganic Chemistry$$c2022$$dQ1
000118290 593__ $$aSpectroscopy$$c2022$$dQ1
000118290 593__ $$aOrganic Chemistry$$c2022$$dQ1
000118290 593__ $$aMolecular Biology$$c2022$$dQ2
000118290 593__ $$aCatalysis$$c2022$$dQ2
000118290 594__ $$a7.8$$b2022
000118290 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000118290 700__ $$aD’amore, S.
000118290 700__ $$aJenkins, B.
000118290 700__ $$aKay, R.
000118290 700__ $$aMurgia, A.
000118290 700__ $$0(orcid)0000-0002-4665-9674$$aHerrera-Marcos, L. V.$$uUniversidad de Zaragoza
000118290 700__ $$aNeun, S.
000118290 700__ $$aSowton, A. P.
000118290 700__ $$aHall, Z.
000118290 700__ $$aPalma-Duran, S. A.
000118290 700__ $$aPalasciano, G.
000118290 700__ $$aReimann, F.
000118290 700__ $$aMurray, A.
000118290 700__ $$aSuppressa, P.
000118290 700__ $$aSabbà, C.
000118290 700__ $$aMoschetta, A.
000118290 700__ $$aKoulman, A.
000118290 700__ $$aGriffin, J. L.
000118290 700__ $$aVacca, M.
000118290 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000118290 773__ $$g23, 12 (2022), 6788 [20 pp.]$$pInt. j. mol. sci.$$tInternational Journal of Molecular Sciences$$x1661-6596
000118290 8564_ $$s4495951$$uhttps://zaguan.unizar.es/record/118290/files/texto_completo.pdf$$yVersión publicada
000118290 8564_ $$s2911946$$uhttps://zaguan.unizar.es/record/118290/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000118290 909CO $$ooai:zaguan.unizar.es:118290$$particulos$$pdriver
000118290 951__ $$a2024-03-18-16:18:43
000118290 980__ $$aARTICLE