000118754 001__ 118754
000118754 005__ 20240319081027.0
000118754 0247_ $$2doi$$a10.1186/s12866-022-02623-w
000118754 0248_ $$2sideral$$a129803
000118754 037__ $$aART-2022-129803
000118754 041__ $$aeng
000118754 100__ $$0(orcid)0000-0003-1210-8732$$aPérez-Rodríguez, Sandra$$uUniversidad de Zaragoza
000118754 245__ $$aA microfluidic-based analysis of 3D macrophage migration after stimulation by Mycobacterium, Salmonella and Escherichia
000118754 260__ $$c2022
000118754 5060_ $$aAccess copy available to the general public$$fUnrestricted
000118754 5203_ $$aMacrophages play an essential role in the process of recognition and containment of microbial infections. These immune cells are recruited to infectious sites to reach and phagocytose pathogens. Specifically, in this article, bacteria from the genus Mycobacterium, Salmonella and Escherichia, were selected to study the directional macrophage movement towards different bacterial fractions. We recreated a three-dimensional environment in a microfluidic device, using a collagen-based hydrogel that simulates the mechanical microarchitecture associated to the Extra Cellular Matrix (ECM). First, we showed that macrophage migration is affected by the collagen concentration of their environment, migrating greater distances at higher velocities with decreasing collagen concentrations. To recreate the infectious microenvironment, macrophages were exposed to lateral gradients of bacterial fractions obtained from the intracellular pathogens M. tuberculosis and S. typhimurium. Our results showed that macrophages migrated directionally, and in a concentration-dependent manner, towards the sites where bacterial fractions are located, suggesting the presence of attractants molecules in all the samples. We confirmed that purified M. tuberculosis antigens, as ESAT-6 and CFP-10, stimulated macrophage recruitment in our device. Finally, we also observed that macrophages migrate towards fractions from non-pathogenic bacteria, such as M. smegmatis and Escherichia coli. In conclusion, our microfluidic device is a useful tool which opens new perspectives to study the recognition of specific antigens by innate immune cells.
000118754 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000118754 590__ $$a4.2$$b2022
000118754 592__ $$a0.937$$b2022
000118754 591__ $$aMICROBIOLOGY$$b53 / 135 = 0.393$$c2022$$dQ2$$eT2
000118754 593__ $$aMicrobiology (medical)$$c2022$$dQ2
000118754 593__ $$aMicrobiology$$c2022$$dQ2
000118754 594__ $$a7.4$$b2022
000118754 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000118754 700__ $$0(orcid)0000-0002-3784-1140$$aBorau, Carlos$$uUniversidad de Zaragoza
000118754 700__ $$0(orcid)0000-0002-9864-7683$$aGarcía-Aznar, José Manuel$$uUniversidad de Zaragoza
000118754 700__ $$0(orcid)0000-0001-8841-6593$$aGonzalo-Asensio, Jesús$$uUniversidad de Zaragoza
000118754 7102_ $$11011$$2630$$aUniversidad de Zaragoza$$bDpto. Microb.Ped.Radio.Sal.Pú.$$cÁrea Microbiología
000118754 7102_ $$15004$$2605$$aUniversidad de Zaragoza$$bDpto. Ingeniería Mecánica$$cÁrea Mec.Med.Cont. y Teor.Est.
000118754 773__ $$g22, 1 (2022), 200 [14 pp.]$$pBMC Microbiol.$$tBMC MICROBIOLOGY$$x1471-2180
000118754 8564_ $$s4670669$$uhttps://zaguan.unizar.es/record/118754/files/texto_completo.pdf$$yVersión publicada
000118754 8564_ $$s2601863$$uhttps://zaguan.unizar.es/record/118754/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000118754 909CO $$ooai:zaguan.unizar.es:118754$$particulos$$pdriver
000118754 951__ $$a2024-03-18-16:52:32
000118754 980__ $$aARTICLE