118792 20260126150156.0 doi 10.1002/jemt.23976 sideral 126938 ART-2022-126938 eng Castiella, T. (orcid)0000-0001-7453-2470 Stromal cells of giant cell tumor of bone show primary cilia in giant cell tumor of bone 2022 Access copy available to the general public Unrestricted Giant cell tumor of bone (GCTB) is a locally aggressive primary bone neoplasm com-posed by tumoral stromal cells (SCs) and a reactive component that consists ofmonocytic/histiocytic cells that give rise by fusion to osteoclast-like multinucleatedcells. Recently, specific Histone 3.3 mutations have been demonstrated in SCs ofGCTB. Many of the pathways related to bone proliferation and regulation depend onthe primary cilium, a microtubule-based organelle that protrudes outside the cell andacts as a sensorial antenna. In the present work, we aimed to study the presence androle of primary cilia in GCTB. Ultrastructural, immunohistochemical, and immunofluo-rescence studies were performed in order to demonstrate, for the first time, that theprimary cilium is located in spindle-shaped SCs of GCTB. Moreover, we showedHedgehog (Hh) signaling pathway activation in these cells. Hence, primary cilia mayplay a relevant role in GCTB tumorogenesis through Hh signaling activation in SCs. info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 2.5 2022 ANATOMY & MORPHOLOGY 5 / 20 = 0.25 2022 Q1 T1 MICROSCOPY 2 / 8 = 0.25 2022 Q1 T1 BIOLOGY 47 / 92 = 0.511 2022 Q3 T2 0.552 2022 Medical Laboratory Technology 2022 Q1 Instrumentation 2022 Q2 Anatomy 2022 Q2 Histology 2022 Q2 5.2 2022 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Iruzubieta, P. Monleón, E. Universidad de Zaragoza (orcid)0000-0002-7453-1766 Cardiel, M. J. Universidad de Zaragoza (orcid)0000-0002-7914-4685 Gómez-Vallejo, J. Universidad de Zaragoza (orcid)0000-0002-5754-6501 Monzón, M. Universidad de Zaragoza (orcid)0000-0002-2787-9671 Junquera, M. C. Universidad de Zaragoza (orcid)0000-0002-9951-1075 1003 443 Universidad de Zaragoza Dpto. Anatom.Histolog.Humanas Area Histología 1013 830 Universidad de Zaragoza Dpto. Cirugía Área Traumatología y Ortopedia 1013 020 Universidad de Zaragoza Dpto. Cirugía Área Anatomía Patológica 85, 3 (2022), 1065-1074 Microsc. res. tech. MICROSCOPY RESEARCH AND TECHNIQUE 1059-910X 5656353 http://zaguan.unizar.es/record/118792/files/texto_completo.pdf Postprint 946389 http://zaguan.unizar.es/record/118792/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:118792 articulos driver 2026-01-26-14:59:55 ARTICLE