Comprehensive and systematic characterization of multi-functionalized cisplatin nano-conjugate: from the chemistry and proteomic biocompatibility to the animal model

Hernández, Ángela-Patricia ; Micaelo, Ania ; Piñol, Rafael ; García-Vaquero, Marina L. ; Aramayona, José J. (Universidad de Zaragoza) ; Criado, Julio J. ; Rodriguez, Emilio ; Sánchez-Gallego, José Ignacio ; Landeira-Viñuela, Alicia ; Juanes-Velasco, Pablo ; Díez, Paula ; Góngora, Rafael ; Jara-Acevedo, Ricardo ; Orfao, Alberto ; Miana-Mena, Javier (Universidad de Zaragoza) ; Muñoz, María Jesús (Universidad de Zaragoza) ; Villanueva, Sergio (Universidad de Zaragoza) ; Millán, Ángel ; Fuentes, Manuel

Resumen: Background
Nowadays, nanoparticles (NPs) have evolved as multifunctional systems combining different custom anchorages which opens a wide range of applications in biomedical research. Thus, their pharmacological involvements require more comprehensive analysis and novel nanodrugs should be characterized by both chemically and biological point of view. Within the wide variety of biocompatible nanosystems, iron oxide nanoparticles (IONPs) present mostly of the required features which make them suitable for multifunctional NPs with many biopharmaceutical applications.

Results
Cisplatin-IONPs and different functionalization stages have been broadly evaluated. The potential application of these nanodrugs in onco-therapies has been assessed by studying in vitro biocompatibility (interactions with environment) by proteomics characterization the determination of protein corona in different proximal fluids (human plasma, rabbit plasma and fetal bovine serum),. Moreover, protein labeling and LC–MS/MS analysis provided more than 4000 proteins de novo synthetized as consequence of the nanodrugs presence defending cell signaling in different tumor cell types (data available via ProteomeXchanges with identified PXD026615). Further in vivo studies have provided a more integrative view of the biopharmaceutical perspectives of IONPs.

Conclusions
Pharmacological proteomic profile different behavior between species and different affinity of protein coating layers (soft and hard corona). Also, intracellular signaling exposed differences between tumor cell lines studied. First approaches in animal model reveal the potential of theses NPs as drug delivery vehicles and confirm cisplatin compounds as strengthened antitumoral agents.

Idioma: Inglés

DOI: 10.1186/s12951-022-01546-y

Año: 2022

Publicado en: Journal of Nanobiotechnology 20 (2022), 341 [19 pp.]
ISSN: 1477-3155

Factor impacto JCR: 10.2 (2022)

Categ. JCR: NANOSCIENCE & NANOTECHNOLOGY rank: 21 / 107 = 0.196 (2022) - Q1 - T1
Categ. JCR: BIOTECHNOLOGY & APPLIED MICROBIOLOGY rank: 12 / 158 = 0.076 (2022) - Q1 - T1

Factor impacto CITESCORE: 10.0 - Engineering (Q1) - Biochemistry, Genetics and Molecular Biology (Q1) - Immunology and Microbiology (Q1) - Pharmacology, Toxicology and Pharmaceutics (Q1) - Chemical Engineering (Q1) - Medicine (Q1)

Factor impacto SCIMAGO: 1.421 - Applied Microbiology and Biotechnology (Q1) - Bioengineering (Q1) - Biomedical Engineering (Q1) - Pharmaceutical Science (Q1) - Molecular Medicine (Q1) - Nanoscience and Nanotechnology (Q1) - Medicine (miscellaneous) (Q1)

Financiación: info:eu-repo/grantAgreement/ES/ISCIII/CB16-12-00400
Financiación: info:eu-repo/grantAgreement/ES/ISCIII-FEDER/PT17-0019-0023
Financiación: info:eu-repo/grantAgreement/ES/ISCIII/FIS/PI14-01538
Financiación: info:eu-repo/grantAgreement/ES/ISCIII/FIS/PI17-01930
Financiación: info:eu-repo/grantAgreement/ES/ISCIII/FIS/PI21-01545

Tipo y forma: Artículo (Versión definitiva)

Área (Departamento): Área Fisiología (Dpto. Farmac.Fisiol.y Med.L.F.)
Área (Departamento): Área Farmacología (Dpto. Farmac.Fisiol.y Med.L.F.)
Área (Departamento): Área Medicina y Cirugía Animal (Dpto. Patología Animal)