118882 20230519145528.0 doi 10.5424/sjar/2021192-17728 sideral 126442 ART-2021-126442 eng Amanzougarene, Z (orcid)0000-0001-8932-4615 Effect of pre-activated Saccharomyces cerevisiae or malate salts on fermentation of ground barley grain under in vitro conditions simulating intensive ruminant feeding 2021 Access copy available to the general public Unrestricted Aim of study: To determine the dose response of Saccharomyces cerevisiae on rumen fermentation of concentrates, and to compare it with the effect of malate salts. Material and methods: S. cerevisiae (0.7, 1.4 and 2.1 mg/g) and malic acid salts (4, 8 or 12 mg/g) were added to barley and compared with barley alone (CTL), in three 24 h in vitro incubation series, using rumen inocula from beef cattle receiving a high concentrate diet. Yeasts were pre-activated by aerobic incubation for 24 h at 30 degrees C. Incubation pH was recorded at 8 and 24 h and volatile fatty acids (VFA) and lactate at 8 h were analysed. Main results: Gas produced with S. cerevisiae was higher than malate (p<0.001). Yeast addition linearly (p<0.01) and quadratically (p<0.05 at 4 h and from 10 to 18 h) increased gas production, but no dose response to malate levels was observed. Dry matter disappearance at 24 h was not affected by S. cerevisiae but increased linearly with malate. Microbial mass linearly increased with the level of yeast (p<0.01) and malate (p=0.09). Adding yeasts did not affect 8 h total VFA concentration compared with CTL, but linear valerate (p<0.01) and butyrate (p=0.092) increases, and a decrease of acetate (p=0.064) were detected. Malate salts linearly increased (p<0.05) total VFA concentration but did not affect VFA proportions. Research highlights: Addition of active yeasts linearly increased barley fermentation and microbial synthesis, whereas the effect of malate salts was of minor magnitude. info:eu-repo/grantAgreement/ES/FEDER/UIDB/04011/2020 info:eu-repo/grantAgreement/ES/MECD/FPU-Formación de profesorado universitario info:eu-repo/grantAgreement/ES/MINECO-DGA-FSE/AGL2013-46820 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 1.233 2021 AGRICULTURE, MULTIDISCIPLINARY 37 / 60 = 0.617 2021 Q3 T2 0.291 2021 Agronomy and Crop Science 2021 Q3 1.9 2021 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Yuste, S (orcid)0000-0003-3927-9793 Fondevila, M Universidad de Zaragoza (orcid)0000-0002-0712-1185 2008 700 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Producción Animal 19, 2 (2021), e06SCO2 [7 pp] Span. j. agric. res. Spanish Journal of Agricultural Research 1695-971X 343365 http://zaguan.unizar.es/record/118882/files/texto_completo.pdf Versión publicada 2529052 http://zaguan.unizar.es/record/118882/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:118882 articulos driver 2023-05-18-15:28:49 ARTICLE