Repositorio Institucional de Documentos

000118902 001__ 118902
000118902 005__ 20240319081004.0
000118902 0247_ $$2doi$$a10.3389/fvets.2022.956630
000118902 0248_ $$2sideral$$a129454
000118902 037__ $$aART-2022-129454
000118902 041__ $$aeng
000118902 100__ $$0(orcid)0000-0001-9907-2941$$aMitjana, Olga$$uUniversidad de Zaragoza
000118902 245__ $$aPhotoperiod and Melatonin Supplementation: Variable Effects on the Quality of Chilled Dog Semen
000118902 260__ $$c2022
000118902 5060_ $$aAccess copy available to the general public$$fUnrestricted
000118902 5203_ $$aThe addition of melatonin in seminal extenders due to its antioxidant properties and its beneficial role in sperm preservation has been previously described, especially in seasonal species. The aim of this study was to study a potential seasonal effect based on photoperiod duration when adding a physiological concentration of melatonin in the canine ejaculate. A total of 24 ejaculates were obtained from 10 healthy dogs during the increasing photoperiod (from December 21 to June 21), whereas 12 ejaculates were collected from five healthy individuals during the decreasing photoperiod (from June 22 to December 20). Each ejaculate was separated into two aliquots, and one of them remained as a control, whereas melatonin (100 pM) was added to the other one (C and M treatment groups, respectively). Diluted semen was refrigerated at 5°C. On days 0, 1, 2, 3, and 6, sperm motility analyses were performed using a CASA system and hypoosmotic swelling test (HOST), osmotic resistance test (ORT), and flow cytometry analysis. No effect of melatonin on motility was detected in either photoperiod. Negative effects of melatonin were found for acrosomal defects, apoptosis, and viability in the decreasing photoperiod. The addition of melatonin to sperm in the decreasing photoperiod could create such a high level that it would cause the described negative effects. We found a beneficial effect of melatonin in the increasing photoperiod on acrosomal defects and apoptosis during 0–6 days. Melatonin treatment also increased viability in the short term (days 1 and 2) for both photoperiods. Also, melatonin can provide certain beneficial effects on mitochondrial activity in the medium term (days 2 and 3) in the decreasing photoperiod.
000118902 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000118902 590__ $$a3.2$$b2022
000118902 592__ $$a0.737$$b2022
000118902 591__ $$aVETERINARY SCIENCES$$b12 / 144 = 0.083$$c2022$$dQ1$$eT1
000118902 593__ $$aVeterinary (miscellaneous)$$c2022$$dQ1
000118902 594__ $$a3.8$$b2022
000118902 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000118902 700__ $$0(orcid)0000-0003-0770-9250$$aAusejo, Raquel$$uUniversidad de Zaragoza
000118902 700__ $$aMendoza, Noelia
000118902 700__ $$aMiguel, Joaquin
000118902 700__ $$0(orcid)0000-0001-5026-5144$$aTejedor, Maria Teresa$$uUniversidad de Zaragoza
000118902 700__ $$aGarrido, Ana Maria$$uUniversidad de Zaragoza
000118902 700__ $$0(orcid)0000-0001-6964-7011$$aFalceto, Maria Victoria$$uUniversidad de Zaragoza
000118902 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000118902 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal
000118902 773__ $$g9 (2022), 956630 [8 pp.]$$pFront. vet. sci.$$tFrontiers in Veterinary Science$$x2297-1769
000118902 8564_ $$s221215$$uhttps://zaguan.unizar.es/record/118902/files/texto_completo.pdf$$yVersión publicada
000118902 8564_ $$s2416045$$uhttps://zaguan.unizar.es/record/118902/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000118902 909CO $$ooai:zaguan.unizar.es:118902$$particulos$$pdriver
000118902 951__ $$a2024-03-18-14:28:42
000118902 980__ $$aARTICLE

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