000118903 001__ 118903
000118903 005__ 20240319081005.0
000118903 0247_ $$2doi$$a10.1007/s00216-022-03900-3
000118903 0248_ $$2sideral$$a129462
000118903 037__ $$aART-2022-129462
000118903 041__ $$aeng
000118903 100__ $$0(orcid)0000-0001-6482-1121$$aMartín-Barreiro, Alba
000118903 245__ $$aGold nanoparticle formation as an indicator of enzymatic methods: colorimetric L-phenylalanine determination
000118903 260__ $$c2022
000118903 5060_ $$aAccess copy available to the general public$$fUnrestricted
000118903 5203_ $$aAn enzymatic-colorimetric method has been developed based on the reaction between l-phenylalanine (l-Phe) and the l-amino acid oxidase (LAAO) in the presence of Au(III), which has led to the formation of gold nanoparticles. The intensity of the localized surface plasmon resonance (LSPR) band of the generated nanoparticles (550 nm) can be related to the concentration of l-Phe in the sample. The mechanism of the LAAO-l-Phe enzyme reaction in the presence of Au(III) has been studied through the evaluation and optimization of experimental conditions. These studies have reinforced the hypothesis that the catalytic center of the enzyme helps the Au(III) reduction and, thanks to the protein, the Au0 form is stabilized as gold nanoparticles (AuNPs). In the calibration study, a sigmoidal relationship between the concentration of the substrate and the LSPR of the nanoparticles was observed. The linearization of the signal has allowed the determination of l-Phe in the range from 17 to 500 µM with an RSD% (150 μM) of 4.8% (n = 3). The method is free of other amino acid interference normally found in blood plasma. These highly competitive results open the possibility of further development of a rapid method for l-Phe determination based on colorimetry.
000118903 536__ $$9info:eu-repo/grantAgreement/ES/DGA/E25-20R$$9info:eu-repo/grantAgreement/ES/MICINN/PID2019-105408GB-I00$$9info:eu-repo/grantAgreement/ES/MINECO/CTQ2016-76846-R
000118903 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000118903 590__ $$a4.3$$b2022
000118903 592__ $$a0.676$$b2022
000118903 591__ $$aCHEMISTRY, ANALYTICAL$$b20 / 86 = 0.233$$c2022$$dQ1$$eT1
000118903 593__ $$aBiochemistry$$c2022$$dQ2
000118903 591__ $$aBIOCHEMICAL RESEARCH METHODS$$b18 / 77 = 0.234$$c2022$$dQ1$$eT1
000118903 593__ $$aAnalytical Chemistry$$c2022$$dQ2
000118903 594__ $$a7.5$$b2022
000118903 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000118903 700__ $$0(orcid)0000-0002-7902-6005$$ade Marcos, Susana$$uUniversidad de Zaragoza
000118903 700__ $$0(orcid)0000-0002-8973-5104$$aGalbán, Javier$$uUniversidad de Zaragoza
000118903 7102_ $$12009$$2750$$aUniversidad de Zaragoza$$bDpto. Química Analítica$$cÁrea Química Analítica
000118903 773__ $$g414, 8 (2022), 2641-2649$$pAnal. Bioanal. Chem.$$tAnalytical and Bioanalytical Chemistry$$x1618-2642
000118903 8564_ $$s2092255$$uhttps://zaguan.unizar.es/record/118903/files/texto_completo.pdf$$yVersión publicada
000118903 8564_ $$s2506561$$uhttps://zaguan.unizar.es/record/118903/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000118903 909CO $$ooai:zaguan.unizar.es:118903$$particulos$$pdriver
000118903 951__ $$a2024-03-18-14:30:58
000118903 980__ $$aARTICLE