118927 20240322114950.0 doi 10.3390/ani12151971 sideral 130042 ART-2022-130042 eng Villanueva Saz, S. Universidad de Zaragoza (orcid)0000-0001-6209-4282 No evidence of SARS-CoV-2 infection in wild mink (Mustela lutreola and neogale vison) from northern Spain during the first two years of pandemic 2022 Access copy available to the general public Unrestricted The impact of the SARS-CoV-2 pandemic on wildlife is largely unevaluated, and extended surveillance of animal species is needed to reach a consensus on the role of animals in the emergence and maintenance of SARS-CoV-2. This infection has been detected in farmed and domestic animals and wild animals, mainly in captivity. The interactions or shared resources with wildlife could represent a potential transmission pathway for the SARS-CoV-2 spill over to other wild species and could lead to health consequences or the establishment of new reservoirs in susceptible hosts. This study evaluated the presence of SARS-CoV-2 in European mink (Mustela lutreola) and American mink (Neogale vison) in Spain by enzyme-linked immunosorbent assay (ELISA) using the receptor binding domain (RBD) of Spike antigen in serum samples and/or by RT-qPCR assays in oropharyngeal and rectal swabs. From January 2020 to February 2022, a total of 162 animals (127 European mink and 35 American mink) with no evidence of SARS-CoV-2 infection were included in the study. Antibodies against the SARS-CoV-2 were not found in the serum samples analysed (n = 126), nor was the virus amplified by RT-qPCR (n = 160 swabs). Our results suggest that the potential role of wild mink and the European mink bred in captivity and released to the wild as dispersers of SARS-CoV-2 is so far low. However, wildlife surveillance for early detection of human and animal risks should be continued. In this sense, epidemiological monitoring measures, including serology and molecular analysis, are necessary. info:eu-repo/grantAgreement/ES/AEI/BFU2016-75633-P info:eu-repo/grantAgreement/ES/AEI/PID2019-105451GB-I00 info:eu-repo/grantAgreement/ES/DGA/E34-R17 info:eu-repo/grantAgreement/ES/DGA-FEDER/B29-17R info:eu-repo/grantAgreement/ES/DGA/LMP58-18 info:eu-repo/grantAgreement/ES/DGA/TB13667 info:eu-repo/grantAgreement/ES/ISCIII/COV20-0030 info:eu-repo/grantAgreement/ES/MCIU-AEI/SAF2017-83120-C2-1-R info:eu-repo/grantAgreement/ES/MINECO/CTQ2013-44367-C2-2-P info:eu-repo/grantAgreement/ES/MITECO/18MNES002 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 3.0 2022 VETERINARY SCIENCES 13 / 144 = 0.09 2022 Q1 T1 AGRICULTURE, DAIRY & ANIMAL SCIENCE 12 / 62 = 0.194 2022 Q1 T1 0.684 2022 Veterinary (miscellaneous) 2022 Q1 Animal Science and Zoology 2022 Q1 4.2 2022 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Giner, J. Palomar, A. M. Gómez, M. A. Põdra, M. Aranda, M. C. Jiménez, M. A. Lizarraga, P. Hernández, R. Portillo, A. Oteo, J. A. Ruíz-Arrondo, I. Universidad de Zaragoza (orcid)0000-0001-8198-8118 Pérez, M. D. Universidad de Zaragoza (orcid)0000-0003-2555-8425 Tobajas, A. P. Universidad de Zaragoza (orcid)0000-0002-2646-5733 Verde, M. Universidad de Zaragoza (orcid)0000-0003-2957-1379 Lacasta, D. Universidad de Zaragoza (orcid)0000-0002-7822-6646 Marteles, D. Hurtado-Guerrero, R. (orcid)0000-0002-3122-9401 Santiago, L. (orcid)0000-0002-1861-5981 Ruiz, H. Universidad de Zaragoza (orcid)0000-0002-8474-2831 Fernández, A. Universidad de Zaragoza (orcid)0000-0002-2557-4890 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 1009 617 Universidad de Zaragoza Dpto. Patología Animal Área Medicina y Cirugía Animal 2008 780 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Tecnología de Alimentos 12, 15 (2022), 1971 [10 pp.] Animals (Basel) Animals 2076-2615 323438 http://zaguan.unizar.es/record/118927/files/texto_completo.pdf Versión publicada 3054492 http://zaguan.unizar.es/record/118927/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:118927 articulos driver 2024-03-22-11:47:35 ARTICLE