000118941 001__ 118941
000118941 005__ 20240319081010.0
000118941 0247_ $$2doi$$a10.3389/fvets.2022.929858
000118941 0248_ $$2sideral$$a130072
000118941 037__ $$aART-2022-130072
000118941 041__ $$aeng
000118941 100__ $$0(orcid)0000-0003-0770-9250$$aAusejo, R.$$uUniversidad de Zaragoza
000118941 245__ $$aNuclear DNA fragmentation in boar spermatozoa: measurement methods and reproductive performance implications
000118941 260__ $$c2022
000118941 5060_ $$aAccess copy available to the general public$$fUnrestricted
000118941 5203_ $$aThe aim of this research was to compare the different techniques to measure sperm nuclear DNA fragmentation (sDF) and to check its relations to boar reproductive value, classical spermiogram parameters, and reproductive results of the doses in sows. Sperm chromatin stability assay (SCSA), terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay, and sperm chromatin dispersion test (SCD, Halomax®) results were compared, finding a statistically significant correlation only between SCSA and TUNEL results. The fertility direct boar effect (DBE) index, calculated from the whole productive life of the boar, was not correlated (p > 0.05) with sDF (measured by any technique). Total or progressive sperm motility was not correlated with sDF, while it found a positive correlation between TUNEL measure and abnormal acrosomes (%) and between SCD measure and total sperm morphological abnormalities (%). No significant correlations were obtained between fertility or prolificacy results and sDF results with the different techniques. However, in the case of total born and SCSA measure, the correlation was close to significance (r partial = -0.095; p = 0.066), appointing to a tendency; as SCSA increases, the number of total piglets born decreases. In conclusion, although the different techniques for the sDF seem not to target exactly the same DNA events and the relationship between their values and the reproductive results and the classical spermiogram results is still to be elucidated, the studied sDF techniques may offer extra information that could be useful for the management of AI studs. Copyright © 2022 Ausejo, Martínez, Mendoza, Bolarin, Tejedor and Falceto.
000118941 536__ $$9info:eu-repo/grantAgreement/ES/UZ/III convocatoria de ayudas para el Desarrollo del programa de doctorados industriales-empresariales
000118941 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000118941 590__ $$a3.2$$b2022
000118941 592__ $$a0.737$$b2022
000118941 591__ $$aVETERINARY SCIENCES$$b12 / 144 = 0.083$$c2022$$dQ1$$eT1
000118941 593__ $$aVeterinary (miscellaneous)$$c2022$$dQ1
000118941 594__ $$a3.8$$b2022
000118941 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000118941 700__ $$0(orcid)0000-0002-8852-9229$$aMartínez, J. M.$$uUniversidad de Zaragoza
000118941 700__ $$aMendoza, N.
000118941 700__ $$aBolarin, A.
000118941 700__ $$0(orcid)0000-0001-5026-5144$$aTejedor, M. T.$$uUniversidad de Zaragoza
000118941 700__ $$0(orcid)0000-0001-6964-7011$$aFalceto, M. V.$$uUniversidad de Zaragoza
000118941 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000118941 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal
000118941 7102_ $$12008$$2780$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Tecnología de Alimentos
000118941 773__ $$g9 (2022), 929858 [11 pp]$$pFront. vet. sci.$$tFrontiers in Veterinary Science$$x2297-1769
000118941 8564_ $$s746239$$uhttps://zaguan.unizar.es/record/118941/files/texto_completo.pdf$$yVersión publicada
000118941 8564_ $$s2344063$$uhttps://zaguan.unizar.es/record/118941/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000118941 909CO $$ooai:zaguan.unizar.es:118941$$particulos$$pdriver
000118941 951__ $$a2024-03-18-15:00:52
000118941 980__ $$aARTICLE