000119022 001__ 119022
000119022 005__ 20240319081027.0
000119022 0247_ $$2doi$$a10.3390/pathogens11030307
000119022 0248_ $$2sideral$$a130289
000119022 037__ $$aART-2022-130289
000119022 041__ $$aeng
000119022 100__ $$aCeballos, Sara
000119022 245__ $$aBeyond CC398: Characterisation of Other Tetracycline and Methicillin-Resistant Staphylococcus aureus Genetic Lineages Circulating in Spanish Hospitals
000119022 260__ $$c2022
000119022 5060_ $$aAccess copy available to the general public$$fUnrestricted
000119022 5203_ $$aTetracycline resistance (TetR) has been evidenced as a good phenotypic marker for detection of livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) isolates of the clonal complex CC398. The aim of this study was to characterise a collection of 95 TetR-MRSA isolates, not belonging to the lineage CC398, that were obtained in a previous multicentre study, to detect other MRSA clonal complexes that could be associated with this phenotypic TetR marker. The TetR-MRSA isolates were recovered from 20 Spanish hospitals during 2016 and they were characterised to determine their antimicrobial resistance and virulence phenotypes/genotypes as well as the presence of the immune evasion cluster (IEC). A high proportion of isolates belonging to the CC1 lineage (46%) were observed, as well as to the CC5, CC8 and CC45 lineages (11% each one). Thirty-two different spa-types were identified, being predominantly CC1-t127 (40%) and CC45-t1081 (11%). The IEC system (with the gene scn as marker) was present in 73% of isolates and 16% produced the Panton Valentine leucocidin (PVL). A high proportion of MRSA-CC1 isolates were scn-negative (38.6%) and 52.9% of them were blaZ-negative. A multidrug resistance (MDR) phenotype was identified in 86% of MRSA isolates. The knowledge of other TetR-MRSA genetic lineages, in addition to CC398, is highly relevant, since most of them were MDR and some of them presented important virulence factors. Strains potentially associated with livestock (as the subpopulation CC1-t127-scn-negative) or with humans (as the CC45 lineage or the subpopulation CC1-scn-positive) have been found in this study. The use of tetracycline-resistance for detection, not only of CC398 but also of other LA-MRSA lineages should be tracked in the future.
000119022 536__ $$9info:eu-repo/grantAgreement/ES/AEI/PID2019-106158RB-I00
000119022 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000119022 590__ $$a3.7$$b2022
000119022 592__ $$a0.807$$b2022
000119022 591__ $$aMICROBIOLOGY$$b62 / 135 = 0.459$$c2022$$dQ2$$eT2
000119022 593__ $$aImmunology and Allergy$$c2022$$dQ2
000119022 593__ $$aImmunology and Microbiology (miscellaneous)$$c2022$$dQ2
000119022 593__ $$aMolecular Biology$$c2022$$dQ2
000119022 593__ $$aMicrobiology (medical)$$c2022$$dQ2
000119022 593__ $$aInfectious Diseases$$c2022$$dQ2
000119022 594__ $$a5.1$$b2022
000119022 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000119022 700__ $$aLozano, Carmen
000119022 700__ $$aAspiroz, Carmen
000119022 700__ $$aRuiz-Ripa, Laura
000119022 700__ $$aEguizábal, Paula
000119022 700__ $$aCampaña-Burguet, Allelen
000119022 700__ $$aCercenado, Emilia
000119022 700__ $$aLópez-Calleja, Ana
000119022 700__ $$0(orcid)0000-0002-2519-701X$$aCastillo, Javier$$uUniversidad de Zaragoza
000119022 700__ $$aAzcona-Gutiérrez, Jose
000119022 700__ $$aTorres, Luis
000119022 700__ $$aCalvo, Jorge
000119022 700__ $$aMartin, Carmen
000119022 700__ $$aNavarro, María
000119022 700__ $$aZarazaga, Myriam
000119022 700__ $$aTorres, Carmen
000119022 700__ $$a
000119022 7102_ $$11011$$2630$$aUniversidad de Zaragoza$$bDpto. Microb.Ped.Radio.Sal.Pú.$$cÁrea Microbiología
000119022 773__ $$g11, 3 (2022), 307 [15 pp.]$$pPathogens$$tPathogens$$x2076-0817
000119022 8564_ $$s2056993$$uhttps://zaguan.unizar.es/record/119022/files/texto_completo.pdf$$yVersión publicada
000119022 8564_ $$s2837493$$uhttps://zaguan.unizar.es/record/119022/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000119022 909CO $$ooai:zaguan.unizar.es:119022$$particulos$$pdriver
000119022 951__ $$a2024-03-18-16:50:58
000119022 980__ $$aARTICLE