000119585 001__ 119585
000119585 005__ 20240122154814.0
000119585 0247_ $$2doi$$a10.1039/d0fo01836h
000119585 0248_ $$2sideral$$a125797
000119585 037__ $$aART-2021-125797
000119585 041__ $$aeng
000119585 100__ $$0(orcid)0000-0002-8100-5596$$aMartínez-Beamonte R.$$uUniversidad de Zaragoza
000119585 245__ $$aDietary squalene modifies plasma lipoproteins and hepatic cholesterol metabolism in rabbits
000119585 260__ $$c2021
000119585 5060_ $$aAccess copy available to the general public$$fUnrestricted
000119585 5203_ $$aTo evaluate the effects of squalene, the main unsaponifiable component of virgin olive oil, on lipid metabolism, two groups of male New Zealand rabbits were fed a 1% sunflower oil-enriched regular diet or the same diet containing 0.5% squalene for 4 weeks. Plasma triglycerides, total- and HDL-cholesterol and their lipoproteins were assayed. Analyses of hepatic lipid droplets, triglycerides, total- and non-esterified cholesterol, squalene, protein and gene expression, and cholesterol precursors were carried out. In the jejunum, the squalene content and mRNA and protein APOB expressions were measured. Finally, we studied the effect of cholesterol precursors in AML12 cells. Squalene administration significantly increased plasma total cholesterol, mainly carried as non-esterified cholesterol in IDL and large LDL, and corresponded to an increased number of APOB100-containing particles without accumulation of triglycerides and decreased reactive oxygen species. Despite no significant changes in the APOB content in the jejunum, the latter displayed increased APOB mRNA and squalene levels. Increases in the amounts of non-esterified cholesterol, squalene, lanosterol, dihydrolanosterol, lathosterol, cholestanol, zymostenol, desmosterol and caspase 1 were also observed in the liver. Incubation of AML12 cells in the presence of lanosterol increased caspase 1. In conclusion, squalene administration in rabbits increases the number of modified APOB-containing lipoproteins, and hepatic cholesterol biosynthesis is linked to caspase 1 probably through lanosterol. © The Royal Society of Chemistry.
000119585 536__ $$9info:eu-repo/grantAgreement/ES/ISCIII-CIBERObn/CB06-03-1012$$9info:eu-repo/grantAgreement/ES/ISCIII-SUDOE/Redvalue-SOE1-PI-E0123$$9info:eu-repo/grantAgreement/ES/MICINN-ISCIIII-FEDER/PID2019-104915RB-I00$$9info:eu-repo/grantAgreement/ES/MICINN/RTI2018-098113-B100$$9info:eu-repo/grantAgreement/ES/MICINN/SAF2016-75441-R
000119585 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000119585 590__ $$a6.317$$b2021
000119585 591__ $$aFOOD SCIENCE & TECHNOLOGY$$b24 / 144 = 0.167$$c2021$$dQ1$$eT1
000119585 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b67 / 297 = 0.226$$c2021$$dQ1$$eT1
000119585 592__ $$a1.011$$b2021
000119585 593__ $$aMedicine (miscellaneous)$$c2021$$dQ1
000119585 593__ $$aFood Science$$c2021$$dQ1
000119585 594__ $$a7.8$$b2021
000119585 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000119585 700__ $$0(orcid)0000-0002-9023-741X$$aSánchez-Marco J.
000119585 700__ $$aFelices M.J.
000119585 700__ $$0(orcid)0000-0002-8442-8041$$aBarranquero C.
000119585 700__ $$aGascón S.$$uUniversidad de Zaragoza
000119585 700__ $$0(orcid)0000-0003-1197-4276$$aArnal C.$$uUniversidad de Zaragoza
000119585 700__ $$aBurillo J.C.
000119585 700__ $$aLasheras R.
000119585 700__ $$aBusto R.
000119585 700__ $$aLasunción M.A.
000119585 700__ $$0(orcid)0000-0002-3595-7668$$aRodríguez-Yoldi M.J.$$uUniversidad de Zaragoza
000119585 700__ $$0(orcid)0000-0002-8251-8457$$aOsada J.$$uUniversidad de Zaragoza
000119585 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000119585 7102_ $$11012$$2410$$aUniversidad de Zaragoza$$bDpto. Farmac.Fisiol.y Med.L.F.$$cÁrea Fisiología
000119585 7102_ $$11009$$2X$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Técnica. Lab. y Talleres
000119585 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000119585 7102_ $$11002$$2050$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Biología Celular
000119585 773__ $$g12, 17 (2021), 8141-8153$$pFOOD & FUNCTION$$tFOOD & FUNCTION$$x2042-6496
000119585 8564_ $$s1527782$$uhttps://zaguan.unizar.es/record/119585/files/texto_completo.pdf$$yPostprint
000119585 8564_ $$s1014204$$uhttps://zaguan.unizar.es/record/119585/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000119585 909CO $$ooai:zaguan.unizar.es:119585$$particulos$$pdriver
000119585 951__ $$a2024-01-22-15:35:22
000119585 980__ $$aARTICLE