000119597 001__ 119597
000119597 005__ 20240516094558.0
000119597 0247_ $$2doi$$a10.1021/acscatal.1c01698
000119597 0248_ $$2sideral$$a127066
000119597 037__ $$aART-2021-127066
000119597 041__ $$aeng
000119597 100__ $$0(orcid)0000-0003-3791-2997$$aGarcía-García A.$$uUniversidad de Zaragoza
000119597 245__ $$aFUT8-directed core fucosylation of N-glycans is regulated by the glycan structure and protein environment
000119597 260__ $$c2021
000119597 5060_ $$aAccess copy available to the general public$$fUnrestricted
000119597 5203_ $$aFUT8 is an essential a-1, 6-fucosyltransferase that fucosylates the innermost GlcNAc of N-glycans, a process called core fucosylation. In vitro, FUT8 exhibits substrate preference for the biantennary complex N-glycan oligosaccharide (G0), but the role of the underlying protein/peptide to which N-glycans are attached remains unclear. Here, we explored the FUT8 enzyme with a series of N-glycan oligosaccharides, N-glycopeptides, and an Asn-linked oligosaccharide. We found that the underlying peptide plays a role in fucosylation of paucimannose (low mannose) and high-mannose N-glycans but not for complex-type N-glycans. Using saturation transfer difference (STD) NMR spectroscopy, we demonstrate that FUT8 recognizes all sugar units of the G0 N-glycan and most of the amino acid residues (Asn-X-Thr) that serve as a recognition sequon for the oligosaccharyltransferase (OST). The largest STD signals were observed in the presence of GDP, suggesting that prior FUT8 binding to GDP-ß-l-fucose (GDP-Fuc) is required for an optimal recognition of N-glycans. We applied genetic engineering of glycosylation capacities in CHO cells to evaluate FUT8 core fucosylation of high-mannose and complex-type N-glycans in cells with a panel of well-characterized therapeutic N-glycoproteins. This confirmed that core fucosylation mainly occurs on complex-type N-glycans, although clearly only at selected glycosites. Eliminating the capacity for complex-type glycosylation in cells (KO mgat1) revealed that glycosites with complex-type N-glycans when converted to high mannose lost the core Fuc. Interestingly, however, for erythropoietin that is uncommon among the tested glycoproteins in efficiently acquiring tetra-antennary N-glycans, two out of three N-glycosites obtained Fuc on the high-mannose N-glycans. An examination of the N-glycosylation sites of several protein crystal structures indicates that core fucosylation is mostly affected by the accessibility and nature of the N-glycan and not by the nature of the underlying peptide sequence. These data have further elucidated the different FUT8 acceptor substrate specificities both in vitro and in vivo in cells, revealing different mechanisms for promoting core fucosylation. ©
000119597 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/RTC-2017-6126-1$$9info:eu-repo/grantAgreement/ES/MINECO/CTQ2017-90039-R$$9info:eu-repo/grantAgreement/ES/MICINN/RTI2018-099592-B-C21$$9info:eu-repo/grantAgreement/ES/MICINN/PID2019-105451GB-I00$$9info:eu-repo/grantAgreement/ES/MICINN/BFU2016-75633-P$$9info:eu-repo/grantAgreement/ES/DGA/LMP58-18$$9info:eu-repo/grantAgreement/ES/DGA-FEDER/E34-R17
000119597 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000119597 590__ $$a13.7$$b2021
000119597 591__ $$aCHEMISTRY, PHYSICAL$$b19 / 165 = 0.115$$c2021$$dQ1$$eT1
000119597 592__ $$a4.202$$b2021
000119597 593__ $$aChemistry (miscellaneous)$$c2021$$dQ1
000119597 593__ $$aCatalysis$$c2021$$dQ1
000119597 594__ $$a20.8$$b2021
000119597 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000119597 700__ $$aSerna S.
000119597 700__ $$aYang Z.
000119597 700__ $$aDelso I.
000119597 700__ $$0(orcid)0000-0001-9224-5854$$aTaleb V.$$uUniversidad de Zaragoza
000119597 700__ $$aHicks T.
000119597 700__ $$aArtschwager R.
000119597 700__ $$aVakhrushev S.Y.
000119597 700__ $$aClausen H.
000119597 700__ $$aAngulo J.
000119597 700__ $$aCorzana F.
000119597 700__ $$aReichardt N.C.
000119597 700__ $$0(orcid)0000-0002-3122-9401$$aHurtado-Guerrero R.
000119597 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000119597 773__ $$g11, 15 (2021), 9052-9065$$pACS catal.$$tACS CATALYSIS$$x2155-5435
000119597 8564_ $$s4926344$$uhttps://zaguan.unizar.es/record/119597/files/texto_completo.pdf$$yVersión publicada
000119597 8564_ $$s3254848$$uhttps://zaguan.unizar.es/record/119597/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000119597 909CO $$ooai:zaguan.unizar.es:119597$$particulos$$pdriver
000119597 951__ $$a2024-05-16-09:33:08
000119597 980__ $$aARTICLE