000119622 001__ 119622
000119622 005__ 20240319081026.0
000119622 0247_ $$2doi$$a10.1016/j.isci.2022.104793
000119622 0248_ $$2sideral$$a129771
000119622 037__ $$aART-2022-129771
000119622 041__ $$aeng
000119622 100__ $$0(orcid)0000-0003-4617-8130$$aAparicio Yuste, Raúl$$uUniversidad de Zaragoza
000119622 245__ $$aBorrelia burgdorferi modulates the physical forces and immunity signaling in endothelial cells
000119622 260__ $$c2022
000119622 5060_ $$aAccess copy available to the general public$$fUnrestricted
000119622 5203_ $$aBorrelia burgdorferi (Bb), a vector-borne bacterial pathogen and the causative agent of Lyme disease, can spread to distant tissues in the human host by traveling in and through monolayers of endothelial cells (ECs) lining the vasculature. To examine whether Bb alters the physical forces of ECs to promote its dissemination, we exposed ECs to Bb and observed a sharp and transient increase in EC traction and intercellular forces, followed by a prolonged decrease in EC motility and physical forces. All variables returned to baseline at 24 h after exposure. RNA sequencing analysis revealed an upregulation of innate immune signaling pathways during early but not late Bb exposure. Exposure of ECs to heat-inactivated Bb recapitulated only the early weakening of EC mechanotransduction. The differential responses to live versus heat-inactivated Bb indicate a tight interplay between innate immune signaling and physical forces in host ECs and suggest their active modulation by Bb.
000119622 536__ $$9info:eu-repo/grantAgreement/EC/H2020/101018587/EU/Individual and Collective Migration of the Immune Cellular System/ICoMICS$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 101018587-ICoMICS$$9info:eu-repo/grantAgreement/ES/MCIU/FPU20-05274$$9info:eu-repo/grantAgreement/ES/MICINN/PID2021-124271OB-I00
000119622 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000119622 590__ $$a5.8$$b2022
000119622 592__ $$a1.624$$b2022
000119622 591__ $$aMULTIDISCIPLINARY SCIENCES$$b15 / 73 = 0.205$$c2022$$dQ1$$eT1
000119622 593__ $$aMultidisciplinary$$c2022$$dQ1
000119622 594__ $$a6.9$$b2022
000119622 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000119622 700__ $$aMuenkel, Marie
000119622 700__ $$aAxarlis, Konstantinos
000119622 700__ $$0(orcid)0000-0002-1878-8997$$aGómez Benito, María J.$$uUniversidad de Zaragoza
000119622 700__ $$aReuss, Annalena
000119622 700__ $$aBlacker, Grace
000119622 700__ $$aTal, Michal Caspi
000119622 700__ $$aKraiczy, Peter
000119622 700__ $$aBastounis, Effie E.
000119622 7102_ $$15004$$2605$$aUniversidad de Zaragoza$$bDpto. Ingeniería Mecánica$$cÁrea Mec.Med.Cont. y Teor.Est.
000119622 773__ $$g25, 8 (2022), 104793 [28 pp.]$$piScience$$tISCIENCE$$x2589-0042
000119622 8564_ $$s6750482$$uhttps://zaguan.unizar.es/record/119622/files/texto_completo.pdf$$yVersión publicada
000119622 8564_ $$s1687807$$uhttps://zaguan.unizar.es/record/119622/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000119622 909CO $$ooai:zaguan.unizar.es:119622$$particulos$$pdriver
000119622 951__ $$a2024-03-18-16:44:50
000119622 980__ $$aARTICLE