119622 20240319081026.0 doi 10.1016/j.isci.2022.104793 sideral 129771 ART-2022-129771 eng Aparicio Yuste, Raúl Universidad de Zaragoza (orcid)0000-0003-4617-8130 Borrelia burgdorferi modulates the physical forces and immunity signaling in endothelial cells 2022 Access copy available to the general public Unrestricted Borrelia burgdorferi (Bb), a vector-borne bacterial pathogen and the causative agent of Lyme disease, can spread to distant tissues in the human host by traveling in and through monolayers of endothelial cells (ECs) lining the vasculature. To examine whether Bb alters the physical forces of ECs to promote its dissemination, we exposed ECs to Bb and observed a sharp and transient increase in EC traction and intercellular forces, followed by a prolonged decrease in EC motility and physical forces. All variables returned to baseline at 24 h after exposure. RNA sequencing analysis revealed an upregulation of innate immune signaling pathways during early but not late Bb exposure. Exposure of ECs to heat-inactivated Bb recapitulated only the early weakening of EC mechanotransduction. The differential responses to live versus heat-inactivated Bb indicate a tight interplay between innate immune signaling and physical forces in host ECs and suggest their active modulation by Bb. info:eu-repo/grantAgreement/EC/H2020/101018587/EU/Individual and Collective Migration of the Immune Cellular System/ICoMICS This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 101018587-ICoMICS info:eu-repo/grantAgreement/ES/MCIU/FPU20-05274 info:eu-repo/grantAgreement/ES/MICINN/PID2021-124271OB-I00 info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 5.8 2022 MULTIDISCIPLINARY SCIENCES 15 / 73 = 0.205 2022 Q1 T1 1.624 2022 Multidisciplinary 2022 Q1 6.9 2022 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Muenkel, Marie Axarlis, Konstantinos Gómez Benito, María J. Universidad de Zaragoza (orcid)0000-0002-1878-8997 Reuss, Annalena Blacker, Grace Tal, Michal Caspi Kraiczy, Peter Bastounis, Effie E. 5004 605 Universidad de Zaragoza Dpto. Ingeniería Mecánica Área Mec.Med.Cont. y Teor.Est. 25, 8 (2022), 104793 [28 pp.] iScience ISCIENCE 2589-0042 6750482 http://zaguan.unizar.es/record/119622/files/texto_completo.pdf Versión publicada 1687807 http://zaguan.unizar.es/record/119622/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:119622 articulos driver 2024-03-18-16:44:50 ARTICLE