000119646 001__ 119646
000119646 005__ 20230519145406.0
000119646 0247_ $$2doi$$a10.1002/vms3.572
000119646 0248_ $$2sideral$$a125835
000119646 037__ $$aART-2021-125835
000119646 041__ $$aeng
000119646 100__ $$0(orcid)0000-0002-2685-1730$$aRodríguez-Largo A.$$uUniversidad de Zaragoza
000119646 245__ $$aDetection of aluminium hydroxide-induced granulomas in sheep by computed tomography: A feasible approach for small ruminant lentiviruses diagnosis and research
000119646 260__ $$c2021
000119646 5060_ $$aAccess copy available to the general public$$fUnrestricted
000119646 5203_ $$aAluminium (Al) hydroxide use as adjuvant induces local formation of long-lasting subcutaneous granulomas in sheep. Macrophages within these granulomas have been identified as a new small ruminant lentivirus (SRLV) replication site in naturally infected animals. Diagnosis of Al hydroxide-induced granulomas in sheep is mostly based on postmortem observations but little information is available on in vivo detection. Computed tomography (CT) is used for studying these reactions in other animal species. To determine if CT could be a tool for in vivo diagnosis and research of subcutaneous Al hydroxide-induced granulomas in sheep. A retrospective survey on thoracic CT scans was performed on 46 adult sheep. Analysis included absence or presence, number and location of subcutaneous nodules. Thoracic CT scans and pathological studies were prescribed to two further sheep. Single or multiple subcutaneous nodules were detected in 26 (56.52%) sheep. One or two nodules per animal were most often observed (36.95%). Size ranged between 1.5 and 4.5 cm. Pre-contrast two-dimensional (2D) CT images showed focal or multifocal increases in subcutaneous tissue thickness. Post-contrast 2D CT images revealed hypointense areas in the centre. Histopathology indicated the presence of granulomas composed by a large number of activated macrophages, surrounding a central core of necrosis. Large intracytoplasmic Al-positive aggregates were demonstrated by lumogallion staining. CT is a useful tool to detect subcutaneous Al hydroxide-induced granulomas in vivo in sheep. CT provides a diagnostic and research tool that can be very useful in future works in Al hydroxide-induced pathology, SRLV infection, or both. © 2021 The Authors. Veterinary Medicine and Science published by John Wiley & Sons Ltd.
000119646 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A17-17R$$9info:eu-repo/grantAgreement/ES/MICINN/RTI2018-096172-B-C33
000119646 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000119646 590__ $$a1.776$$b2021
000119646 591__ $$aVETERINARY SCIENCES$$b61 / 145 = 0.421$$c2021$$dQ2$$eT2
000119646 592__ $$a0.427$$b2021
000119646 593__ $$aVeterinary (miscellaneous)$$c2021$$dQ2
000119646 594__ $$a1.8$$b2021
000119646 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000119646 700__ $$aCastells E.
000119646 700__ $$ade Miguel R.
000119646 700__ $$aGómez Á.
000119646 700__ $$0(orcid)0000-0002-8474-2831$$aRuiz H.$$uUniversidad de Zaragoza
000119646 700__ $$0(orcid)0000-0002-8133-2124$$aPérez M.$$uUniversidad de Zaragoza
000119646 700__ $$aReina R.
000119646 700__ $$0(orcid)0000-0003-0042-8800$$aFerrer L.M.$$uUniversidad de Zaragoza
000119646 700__ $$0(orcid)0000-0002-2053-9842$$aLuján L.$$uUniversidad de Zaragoza
000119646 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000119646 7102_ $$11001$$2025$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Anatom.Anatom.Patológ.Com
000119646 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal
000119646 773__ $$g7, 5 (2021), 1757-1761$$pVet. med. sci.$$tVeterinary medicine and science$$x2053-1095
000119646 8564_ $$s3067905$$uhttps://zaguan.unizar.es/record/119646/files/texto_completo.pdf$$yVersión publicada
000119646 8564_ $$s2094553$$uhttps://zaguan.unizar.es/record/119646/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000119646 909CO $$ooai:zaguan.unizar.es:119646$$particulos$$pdriver
000119646 951__ $$a2023-05-18-13:47:56
000119646 980__ $$aARTICLE